Determination of metformin and other biguanides in forensic whole blood samples by hydrophilic interaction liquid chromatography–electrospray tandem mass spectrometry

Biomedical Chromatography - Tập 26 Số 1 - Trang 1-5 - 2012
Lambert K. Sørensen1
1Section for Toxicology and Drug Analysis, Department of Forensic Medicine, Aarhus University, Brendstrupgaardsvej 100, 8200 Aarhus N, Denmark

Tóm tắt

ABSTRACTMetformin is an anti‐diabetic drug in the biguanide class which also includes phenformin and buformin. Because of the potential adverse effects of the biguanides, a reliable liquid chromatography–tandem mass spectrometry method using pneumatically assisted electrospray ionization was developed for the quantification of the drugs in both live and post‐mortem human whole blood. The blood proteins were precipitated by the addition of a mixture of methanol and acetonitrile, and the extract was cleaned up by cation‐exchange solid‐phase extraction to eliminate ion suppression effects. The separation was performed by hydrophilic interaction liquid chromatography. Matrix‐matched calibrants combined with isotope dilution of metformin were used for calibration. The detection limits were 0.01 mg/L for metformin and phenformin and the relative intra‐laboratory reproducibility standard deviations were less than 6% at concentrations of 1–10 mg/L. The mean true recoveries were greater than 86%.

Từ khóa


Tài liệu tham khảo

10.1016/j.jchromb.2003.12.017

10.1002/bmc.723

10.1016/j.jchromb.2007.04.032

10.1016/j.jpba.2003.11.017

10.1021/ac00238a002

ISO.Standard 5725‐2. Accuracy (Trueness and Precision) of Measurement Methods and Results – Part 2: Basic Method for the Determination of Repeatability and Reproducibility of a Standard Measurement Method.International Organization for Standardization:Geneva 1994.

10.1016/j.jpba.2004.09.007

10.1016/j.jchromb.2009.09.020

10.1016/j.jpba.2007.06.003

The International Association of Forensic Toxicologists.Reference Blood Level List of Therapeutic and Toxic Substances. Available from:http:/www.tiaft.org(accessed 8 December2010).

10.1016/j.jchromb.2004.05.006

10.1016/j.jchromb.2007.04.002

Thông tin
Thông tin xuất bản

Biomedical Chromatography

Tập 26 Số 1

1-5

Thông tin tác giả