Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set

Altschul, 1990, Basic local alignment search tool, J Mol Biol, 215, 403, 10.1016/S0022-2836(05)80360-2

Amann, 1995, Phylogenetic identification and in situ detection of individual microbial cells without cultivation, Microbiol Rev, 59, 143, 10.1128/MMBR.59.1.143-169.1995

Attfield, 1999, Applications of flow cytometry to microbiology of food and beverage industries, Australas Biotechnol, 9, 159

Blok, 1997, Quantitative analysis of 16S rDNA using competitive PCR and the QPCR system 5000, Biotechniques, 22, 700, 10.2144/97224st05

Bottger, 1990, Frequent contamination of Taq polymerase with DNA, Clin Chem, 36, 1258, 10.1093/clinchem/36.6.1258b

Corless, 2000, Contamination and sensitivity issues with a real-time universal 16S rRNA PCR, J Clin Microbiol, 38, 1747, 10.1128/JCM.38.5.1747-1752.2000

Dymock, 1996, Molecular analysis of microflora associated with dentoalveolar abscesses, J Clin Microbiol, 34, 537, 10.1128/JCM.34.3.537-542.1996

Farelly, 1995, Effect of genome size and rrn gene copy number on PCR amplification of 16S rRNA genes from a mixture of bacterial species, Appl Environ Microbiol, 61, 2798, 10.1128/AEM.61.7.2798-2801.1995

Gough, 1983, Sequence diversity among related genes for recognition of specific targets in DNA molecules, J Mol Biol, 166, 1, 10.1016/S0022-2836(83)80047-3

Greisen, 1994, PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid, J Clin Microbiol, 32, 335, 10.1128/JCM.32.2.335-351.1994

Gurtler, 1996, New approaches to typing and identification of bacteria using 16S–23S rDNA spacer region, Microbiology, 142, 3, 10.1099/13500872-142-1-3

Heid, 1996, Real time quantitative PCR, Genome Res, 6, 986, 10.1101/gr.6.10.986

Holt, 1994, Bergey’s Manual of Determinative Bacteriology

Hugenholtz, 1998, Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity, J Bacteriol, 180, 4765, 10.1128/JB.180.18.4765-4774.1998

Johnson, 1995, Techniques for controlling variability in Gram staining of obligate anaerobes, J Clin Microbiol, 33, 755, 10.1128/JCM.33.3.755-758.1995

Klappenbach, 2000, rRNA operon copy number reflects ecological strategies of bacteria, Appl Environ Microbiol, 66, 1328, 10.1128/AEM.66.4.1328-1333.2000

Klausegger, 1999, Gram type specific broad-range PCR amplification for rapid detection of 62 pathogenic bacteria, J Clin Microbiol, 37, 464, 10.1128/JCM.37.2.464-466.1999

Kroes, 1999, Bacterial diversity within the human subgingival crevice, Proc Natl Acad Sci USA, 96, 14547, 10.1073/pnas.96.25.14547

Lyons, 2000, Quantitative real-time PCR for Porphyromonas gingivalis and total bacteria, J Clin Microbiol, 38, 2362, 10.1128/JCM.38.6.2362-2365.2000

Marchesi, 1998, Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA, Appl Environ Microbiol, 64, 795, 10.1128/AEM.64.2.795-799.1998

Massey, 1993, The association of carious dentine microflora with tissue changes in human pulpitis, Oral Microbiol Immunol, 8, 30, 10.1111/j.1399-302X.1993.tb00539.x

Miller, 1972, Experiments in Molecular Genetics

Neidhardt, 1990, Physiology of the Bacterial Cell. A Molecular Approach, 389

Relman, 1992, Identification of the uncultured Bacillus of Whipple’s disease, New Eng J Med, 327, 293, 10.1056/NEJM199207303270501

Rupf, 1999, Quantification of bacteria in oral samples by competitive polymerase chain reaction, J Dent Res, 78, 850, 10.1177/00220345990780040501

Schmidt, 1991, Detection of DNA contamination in Taq polymerase, Biotechniques, 11, 176

Suzuki, 2000, Quantitative analysis of small-subunit rRNA genes in mixed microbial populations via 5′-nuclease assays, Appl Environ Microbiol, 66, 4605, 10.1128/AEM.66.11.4605-4614.2000

Syed, 1972, Survival of human dental plaque flora in various transport media, Appl Microbiol, 26, 459, 10.1128/AEM.26.4.459-465.1973

Tao, 1993, Transformation efficiency of EMS-induced mutants of Streptococcus mutans of altered cell shape, J Dent Res, 72, 1032, 10.1177/00220345930720060701

1982, Media for the isolation, characterization and identification of obligately anaerobic bacteria

Veal, 2000, Fluorescence staining and flow cytometry for monitoring microbial cells, J Immunol Methods, 243, 191, 10.1016/S0022-1759(00)00234-9

Ward, 1990, 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community, Nature, 345, 63, 10.1038/345063a0

Wilson, 1990, Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction, J Clin Microbiol, 28, 1942, 10.1128/JCM.28.9.1942-1946.1990

Wintzingerode, 1997, Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis, FEMS Microbiol Rev, 21, 213, 10.1111/j.1574-6976.1997.tb00351.x

Yanisch-Perron, 1985, Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors, Gene, 33, 103, 10.1016/0378-1119(85)90120-9

Zerr, 1998, Effect of red blood cells on the growth of Porphyromonas endodontalis and microbial community development, Oral Microbiol Immunol, 13, 106, 10.1111/j.1399-302X.1998.tb00720.x