Determination of 12 pueraria components by high‐performance liquid chromatography‐mass spectrometry
Tóm tắt
Puerariae radix, a commonly used Chinese herb drug derived from the dried root of legume plant, contains a series of isoflavones as its chief pharmacologically active constituents. Using 12 pueraria components as markers, an LC‐UV‐MS method requiring less than 60 min, was developed for estimating the quality of pueraria samples within 60 min. Extracts were analyzed using a Cosmosil 5C18‐MS column, by gradient elution with an aqueous solution of acetic acid and methanol–ACN at a flow‐rate of 1.0 mL/min. Peaks were detected at 254 nm and each peak was identified by LC/MS. The reproducibilities (RSD) of this proposed method, on the basis of peak‐area ratios from six replicate injections, were 0.93–1.42% (intraday) and 1.05–1.63% (interday) at a detection limit of 0.12–0.78 μg/mL. Most of the markers used in this study can be classified, respectively, into three major categories, namely, isoflavones,
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