Detection of multiple paternity in the Kemp's ridley sea turtle with limited sampling

Molecular Ecology - Tập 8 Số 5 - Trang 819-830 - 1999
Kichler1, Mark T. Holder2, J.R. Davis3, Márquez‐M4, Julie A. Owens1
1Department of Biology, Texas A & M University, College Station, TX, 77845, USA.
2Department of Zoology, University of Texas at Austin, Austin, TX 78712, USA
3Department of Animal Science, Texas A&M University, College Station, TX 77845, USA
4Instituto Nacional de la Pesca, Apartado Postal 591, Manzanillo, Colima 28200, México

Tóm tắt

An important question in sea turtle biology is the number of males that contribute to the fertilization of a clutch of eggs. Previous studies on other sea turtle species have indicated little to no multiple paternity. We conclude here that female Kemp's ridleys, Lepidochelys kempi, are polyandrous. DNA from 26 mother and offspring groups was analysed at three microsatellite loci to identify paternal alleles. Three paternal alleles were observed among 14 of the clutches; four paternal alleles were observed among the offspring of an additional female. A maximum likelihood analysis not only rejects the model of single paternity, but also rejects the model of equal paternal contribution to the clutch. By explicitly addressing the high mutation rate of microsatellite markers, our analysis rejected mutation as the sole cause of multiple paternal alleles.

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Tài liệu tham khảo

Alvarado J, 1991, Comportamiento reproductivo de la tortuga negra Chelonia agassizi., Ciencia Y Desarollo, 17, 43

BirkheadTR&MøllerAP(1992)Sperm Competition in Birds: Evolutionary Causes and Consequences.Academic Press London.

10.1111/j.1095-8312.1993.tb00933.x

10.1016/S0003-3472(72)80155-6

Carr AF, 1963, Panspecific reproductive convergence in Lepidochelys kempi., Ergebnisse der Biologie, 26, 298

Comuzzie DKC, 1990, A quantitative analysis of courtship behavior in captive green sea turtles (Chelonia mydas)., Herpetologica, 46, 195

CurtisC WilliamsCJ SpotilaJR((in press)) Mating system of Caribbean leatherback turtles as indicated by analysis of microsatellite DNA from hatchlings and adult females. In:Proceedings of the 18th International Symposium on Sea Turtle Biology and Conservation.NOAA Technical Memorandum.

DuttonPH(1996) Methods for collection and preservation of samples for sea turtle genetic studies. In: (eds Bowen BW Witzell WN )Proceedings of the International Symposium on Sea Turtle Conservation and Genetics pp. 17–24. NOAA Technical Memorandum NMFS‐SEFSC‐396.

DuttonP((in press)) Tendency toward single paternity in leatherbacks detected with microsatellites. In:Proceedings of the 18th International Symposium on Sea Turtle Biology and Conservation. NOAA Technical Memorandum.

EhrhartLM(1982) A review of sea turtle reproduction. In: (ed. Bjorndal K)Biology and Conservation of Sea Turtles pp. 29–38. Smithsonian Institution Press Washington.

10.2307/1382825

10.2307/1437776

10.1046/j.1365-294x.1998.00355.x

FitzSimmons NN, 1995, Conservation and dynamics of microsatellite loci over 300 million years of marine turtle evolution., Molecular Biology and Evolution, 12, 432

Gist DH, 1987, Storage of sperm in the reptilian oviduct., Scanning Microscopy, 1, 1839

10.1093/oxfordjournals.jhered.a110480

10.1111/j.1558-5646.1986.tb05751.x

10.1111/j.1365-294X.1993.tb00102.x

10.1071/WR9930513

Márquez‐MR(1994)Synopsis of Biological Data on the Kemp's Ridley Turtle Lepidochelys kempi(Garman 1880).NOAA Technical Memorandum NMFS‐SEFSC‐343.

Mendonça MT, 1986, Offshore movements of post‐nesting Kemp's ridley sea turtles (Lepidochelys kempi)., Herpetologica, 42, 373

10.1093/icb/20.3.549

10.1093/oxfordjournals.jhered.a111573

RostalDC(1991) The reproductive behavior and physiology of the Kemp's ridley sea turtle Lepidochelys kempi(Garman 1880). PhD Thesis Texas A & M University.

SambrookJ FritschEF ManiatisT(1989)Molecular Cloning 2nd edn. Cold Spring Harbor Laboratory Press Cold Spring Harbor New York.

SchlöttererC&PembertonJ(1994) The use of microsatellites for genetic analysis of natural populations. In: (eds Schierwater B Streit B Wagner GP DeSalle R)Molecular Ecology and Evolution: Approaches and Applications pp. 203–214. Birkhäuser Basel.

SilveySD(1970)Statistical Inference. Penguin Books Baltimore.

Wood JR, 1988, Captive reproduction of Kemp's ridley Lepidochelys kempi., Herpetological Journal, 1, 247

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Thông tin xuất bản

Molecular Ecology

Tập 8 Số 5

819-830

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