Detection of human immunodeficiency virus, hepatitis C virus, and hepatitis B virus in postmortem blood specimens using infectious disease assays licensed for cadaveric donor screening

Transplant Infectious Disease - Tập 20 Số 1 - 2018
Melissa A. Greenwald1, Stephen Kerby2, Kori Francis2, Anna C. Noller3, William T. Gormley3, Robin Biswas2, Richard A. Forshee4
1Division of Transplantation Health Resources and Services Administration Rockville MD USA
2US Food and Drug Administration, Silver Spring, MD, USA
3Virginia Office of the Chief Medical Examiner Richmond VA USA
4Office of Biostatistics and Epidemiology US Food and Drug Administration Silver Spring MD USA

Tóm tắt

AbstractBackground

Evaluation of assay performance on postmortem blood specimens (obtained after cessation of the heartbeat) presents unique scientific and regulatory challenges. In the United States, assay performance is evaluated in part by spiking postmortem specimens.

Methods

Fifty‐four specimens obtained from decedents known to be infected with human immunodeficiency virus (HIV), hepatitis C virus (HCV), or hepatitis B virus (HBV), including some coinfections, were tested for each virus using Food and Drug Administration (FDA)‐licensed donor screening tests for nucleic acid, antibody, and antigen.

Results

For each disease, >95% of subjects who were reported to have an infection at the time of death had a positive test result on at least one of the donor screening assays for that infection.

Conclusion

Licensed donor screening tests were positive on postmortem specimens obtained within 24 hours of death from individuals dying with HIV, HCV, and/or HBV, and were able to detect presence of the virus. The use of multiple tests (including antibody and direct viral detection methods) is necessary to adequately evaluate donors.

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