Detection of Circulating Tumor DNA in Early- and Late-Stage Human Malignancies

Chetan Bettegowda1,2, Mark Sausen3, Rebecca J. Leary3, Isaac Kinde3, Yuxuan Wang3, Nishant Agrawal1,2, Bjarne R. Bartlett4,5, Hao Wang3, Brandon Luber3, Rhoda M. Alani6, Emmanuel S. Antonarakis3, Nilofer S. Azad3, Alberto Bardelli7,8,9, Henry Brem1, John L. Cameron1, Clarence Lee10, Leslie A. Fecher11,12, Gary L. Gallia1, Peter Gibbs13,14, Dung T. Le4,5, Robert Giuntoli1, Michael Goggins1, Michael D. Hogarty15, Matthias Holdhoff3, Seung‐Mo Hong16,17, Yuchen Jiao3, Hartmut Juhl18, Jenny J. Kim3, Giulia Siravegna19, Daniel A. Laheru3, Calogero Lauricella19, Michael Lim1, Evan J. Lipson3, Suely Kazue Nagahashi Marie20, George J. Netto1, Kelly S. Oliner21, Alessandro Olivi1, Louise Olsson22, Gregory J. Riggins1, Andrea Sartore‐Bianchi19, Kerstin Schmidt3, le-Ming Shih1, Sueli Mieko Oba‐Shinjo20, Salvatore Siena19, Dan Theodorescu23, Jeanne Tie24, Timothy T. Harkins10, Silvio Veronese19, Tian‐Li Wang1, Jon Weingart1, Christopher L. Wolfgang1, Laura D. Wood1, Dongmei Xing1, Ralph H. Hruban1, Jian Wu25,26, Peter J. Allen27, C. Max Schmidt28, Michael A. Choti1, Victor E. Velculescu3, Kenneth W. Kinzler3, Bert Vogelstein3, Nickolas Papadopoulos3, Luis A. Díaz4,5
1Departments of Surgery, Medicine, Pathology, Obstetrics and Gynecology, Otolaryngology, and Neurosurgery at Johns Hopkins, Baltimore, MD 21231, USA.
2Departments of Surgery, Medicine, Pathology, Obstetrics and Gynecology, Otolaryngology, and Neurosurgery at Johns Hopkins, Baltimore, MD 21231, USA.; Ludwig Center for Cancer Genetics and Therapeutics, Howard Hughes Medical Institute and the Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA.
3Ludwig Center for Cancer Genetics and Therapeutics, Howard Hughes Medical Institute and the Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA.
4Ludwig Center for Cancer Genetics and Therapeutics, Howard Hughes Medical Institute and the Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA.; Swim Across America Laboratory at Johns Hopkins, Baltimore, MD 21231, USA.
5Swim Across America Laboratory at Johns Hopkins, Baltimore, MD 21231, USA.
6Department of Dermatology, Boston University, Boston, MA 02215, USA.
7Department of Oncology, University of Torino, Candiolo, Turin 10060, Italy.
8Department of Oncology, University of Torino, Candiolo, Turin 10060, Italy.; FIRC Institute of Molecular Oncology (IFOM), Milan 20139, Italy.; Institute for Cancer Research and Treatment at Candiolo, University of Torino, Candiolo, Turin 10060, Italy.
9FIRC Institute of Molecular Oncology (IFOM), Milan 20139, Italy.
10Advanced Applications and Collaborations, Life Technologies, Foster City, CA 94404, USA.
11Division of Oncology, University of Indiana, Indianapolis, IN 46202, USA.; Indiana University Health, Indianapolis, IN 46202, USA.
12Indiana University Health, Indianapolis, IN 46202, USA.
13Ludwig Institute for Cancer Research, Melbourne Branch, Royal Melbourne Hospital, Melbourne, Victoria 3084, Australia.; Western Hospital, Melbourne, Victoria 3011, Australia.
14Western Hospital, Melbourne, Victoria 3011, Australia.
15Division of Oncology, The Children’s Hospital of Philadelphia and the Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
16Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea
17Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea.; Departments of Surgery, Medicine, Pathology, Obstetrics and Gynecology, Otolaryngology, and Neurosurgery at Johns Hopkins, Baltimore, MD 21231, USA.
18Indivumed GmbH, Hamburg 20251, Germany.
19Niguarda Cancer Center, Ospedale Niguarda Ca’ Granda, Milan 20162, Italy.
20Department of Neurology and Pathology, School of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
21Amgen Inc., Thousand Oaks, CA 91320, USA
22Department of Molecular Medicine and Surgery, Karolinska Institute, Stockholm SE-171 76, Sweden.
23University of Colorado Comprehensive Cancer Center, Aurora, CO, 80045, USA
24Ludwig Institute for Cancer Research, Melbourne Branch, Royal Melbourne Hospital, Melbourne, Victoria 3084, Australia.
25Ludwig Center for Cancer Genetics and Therapeutics, Howard Hughes Medical Institute and the Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA.; MyGenostics Inc., 801 West Baltimore Street, Baltimore, MD 21205, USA.
26MyGenostics Inc., 801 West Baltimore Street, Baltimore, MD 21205, USA.
27Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA
28Departments of Surgery, Biochemistry, and Molecular Biology, Indiana University, Indianapolis, IN 46202, USA.

Tóm tắt

Circulating tumor DNA can be used in a variety of clinical and investigational settings across tumor types and stages for screening, diagnosis, and identifying mutations responsible for therapeutic response and drug resistance.

Từ khóa


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