Detection and characterization of Shiga toxin-producingEscherichia coliin faeces and lymphatic tissue of free-ranging deer

Epidemiology and Infection - Tập 141 Số 2 - Trang 251-259 - 2013
Marlene Eggert1, Elisabeth Stüber2, Marco Heurich3, Maria Fredriksson‐Ahomaa4, Ylanna Kelner Burgos5, Lothar Beutin5, Erwin Märtlbauer2
1Institute of Human Genetics, University Hospital, Ludwig Maximilians University, Munich, Germany
2Institute of Food Science, Department of Veterinary Sciences, Faculty of Veterinary Medicine, Ludwig-Maximilians-University Munich, Germany
3Department of Research and Documentation, Bavarian Forest National Park, Grafenau, Germany
4Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Finland
5National Reference Laboratory for Escherichia coli, Federal Institute for Risk Assessment (BfR), Berlin, Germany

Tóm tắt

SUMMARYShiga toxin-producingEscherichia coli(STEC) have led to outbreaks worldwide and are considered emerging pathogens. Infections by STEC in humans have been reported after consumption of mainly beef, but also deer. This study investigated the occurrence of STEC in deer in Germany. The virulence geneseae,e-hlyAandsaa, thestxsubtypes, pulsed-field gel electrophoresis (PFGE) patterns and serovars were studied. In total, 120 samples of 60 animals were screened by real-time polymerase chain reaction (PCR). The PCR results showed a high detection rate ofstxgenes (83%). Mainly faecal samples, but also some lymphatic tissue samples, testedstx-positive. All isolates carriedstx2, wereeae-negative and carriede-hlyAin 38% andsaain 9% of samples. Serovars (O88:[H8], O174:[H8], O146:H28) associated with human diseases were also identified. In some animals, isolates from lymphatic tissue and faecal samples showed undistinguishable PFGE patterns. The examined deer were shown to be relevant reservoirs of STEC with subtypestx2bpredominating.

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Tài liệu tham khảo

10.7589/0090-3558-37.4.755

10.1089/fpd.2008.0188

10.1111/j.1348-0421.1998.tb02356.x

10.1086/338115

10.1186/1751-0147-48-7

Lim, 2010, A brief overview of Escherichia coli O157:H7 and its plasmid O157, Journal of Microbiology and Biotechnology, 20, 5, 10.4014/jmb.0908.08007

10.1128/IAI.69.11.6999-7009.2001

10.11150/kansenshogakuzasshi1970.73.1140

10.1128/JCM.39.11.3992-3998.2001

10.1128/JCM.41.7.2884-2893.2003

Heurich, 2011, Management and Conservation of large mammals in the Bavarian Forest National Park, Silva Gabreta, 17, 1

10.1128/JCM.42.3.1099-1108.2004

Pichner, 2005, Occurrence of Salmonella spp. and shigatoxin-producing Escherichia coli (STEC) in horse faeces and horse meat products [in German], Berliner und Münchener Tierärztliche Wochenschrift, 118, 321

10.1016/j.tvjl.2008.01.011

10.1128/JCM.41.5.2106-2112.2003

Wells, 1991, Localization of translocating Escherichia coli, Proteus mirabilis, and Enterococcus faecalis within cecal and colonic tissues of monoassociated mice, Infection and Immunity, 59, 4693, 10.1128/IAI.59.12.4693-4697.1991

World Health Organisation. Zoonotic non-O157 shiga toxin-producing Escherichia coli (STEC) Report of a WHO Scientific working Group Meeting, 1998, Berlin, 23–26 June 1998.

Lehmann, 2006, Detection of STEC in faecal samples of free-ranging wild and in wild meat samples [in German], Fleischwirtschaft, 4, 93

10.1016/j.jpeds.2009.02.051

10.2903/j.efsa.2011.2090

10.1186/1751-0147-46-23

10.1017/S0950268899004094

Robert Koch-Institut (RKI). SurvStat@RKI database. Surveillance status of EHEC/STEC listed by Serovars reported from 2009–2011 (http://www3.rki.de/SurvStat/QueryForm.aspx). Accessed 15 September 2011.

10.1111/j.1365-2672.2010.04855.x

10.1128/JCM.42.2.645-651.2004

10.1016/j.vetmic.2006.12.012

10.1001/jama.1997.03540390059036

10.3201/eid0805.010373

10.1016/j.ijmm.2005.07.010

10.4315/0362-028X-70.6.1493

10.1016/S0140-6736(10)60963-4

10.1080/10408410601172172

10.1016/S0378-1135(03)00039-7

10.1128/AEM.00370-11

10.1016/j.vetmic.2006.07.006

10.1128/AEM.00904-09

10.1128/IAI.70.12.6788-6797.2002

10.1128/AEM.00873-07

10.1128/AEM.00275-07

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Epidemiology and Infection

Tập 141 Số 2

251-259

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