Design of minimally invasive optical imaging probes to track vascular gene expression: a Monte Carlo simulation analysis

A. Kumar1, H.H. Chen1, E. Long1, Xiaoming Yang2,1
1Depments of Radiology and Biomedical Engineering, School of Medicine, Johns Hopkins University, Baltimore, MD, USA
2JHOC, Radiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA

Tóm tắt

A Monte Carlo simulation analysis of optical probe design for minimally invasive tracking of gene expression in the vasculature was performed. The simulations based on efficient light delivery for excitation of green fluorescent protein (GFP) marker revealed the possibility of using percutaneous probes and intravascular probes to detect gene expression in the vessel walls. A percutaneous probe of 5 mm diameter and an intravascular probe of 2 mm diameter produced identical fluence distribution in the media-intima interface of a diseased aorta for optimal excitation of GFP. But there was a seven-fold increase in the fluence rate in the normal aorta by the intravascular probe.

Từ khóa

#Optical design #Minimally invasive surgery #Optical imaging #Probes #Gene expression #Monte Carlo methods #Optical scattering #Biomedical optical imaging #Optical attenuators #Fluorescence

Tài liệu tham khảo

10.1364/AO.37.007429 chen, 2001, Development of a non-invasi ve optical imaging method for tracking vascular gene expression, Proceeding Book IEEE Engineering in Medicine and Biology Society, 5.5.2?4 10.1016/0169-2607(95)01640-F 10.1016/S0169-2607(97)00021-7 10.1364/AO.28.004286 oraevsky, 1988, Spectral properties of human atherosclerotic blood vessel walls, Laser Life Sci, 2, 275 10.1002/lsm.1900090210 10.1148/radiographics.18.6.9821188 10.3810/hp.1999.06.147 10.1073/pnas.97.3.1206 moritz, 1999, Fluorescent photoreceptors of transgenic Xenopus laevis imaged in vivo by two microscopy techniques, Invest Ophthalmol Vis Sci, 40, 3276 10.1148/radiology.219.1.r01ap23171 10.1038/sj.gt.3300579 10.1161/01.CIR.91.2.541 2001, heart and stroke statistical update kumar, 0, Optical imaging of green fluorescent marker proteins for tracking vascular gene expression: A feasibility study in human tissue-like phantoms, Proceedings of SPIE Photonics West 4623-51