Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA
Tóm tắt
We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a variety of bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecological and systematic studies than PCR amplimers that are currently more generally used.
Từ khóa
Tài liệu tham khảo
Ausubel F. M. Brent R. Kingston R. E. Moore D. D. Seidman J. G. Smith J. A. Struhl K. Current protocols in molecular biology. 1989 Greene Publishing Associates-Wiley Interscience New York N.Y
Baranton G. Personal communication.
Lane D. J. 16S/23S rRNA sequencing Nucleic acid techniques in bacterial systematics. Stackebrandt E. Goodfellow M. 1991 115 175 John Wiley & Sons Chichester United Kingdom
Parkes R. J. Cragg B. A. Fry J. C. Herbert R. A. Wimpenny J. W. T. Bacterial biomass and activity in deep sediment layers from the Peru margin. Philos. Trans. R. Soc. Lond. B 133 1990 139 153
Rochelle P. A. Will J. A. K. Fry J. C. Jenkins G. J. S. Parkes R. J. Turley C. M. Weightman A. J. Extraction and amplification of 16S rRNA genes from deep marine sediments and seawater to assess bacterial community diversity Nucleic acids in the environment: methods and applications. van Elsas J. D. Trevors J. T. 1995 219 239 Springer-Verlag Berlin Germany
Sambrook J. Fritsch E. F. Maniatis T. Molecular cloning: a laboratory manual 2nd ed. 1989 Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y