Richard E. Spencer1,2, Fernando B Toledo1,2, B T Williams1,2, Norma L. Yoss1,2
1Mercy Hospital Laboratory, Urbana, Ill. 61801
2Mercy Hospital Laboratory, Urbana, Ill. 61801; and the School of Chemical Sciences, Department of Biochemistry, University of Illinois, Urbana, Ill. 61801 (R.D.S.)
Tóm tắt
Abstract
We have constructed and automated a flow cell polarization fluorometer and demonstrated two specific clinical applications of fluorescence polarization assay, i.e., the enzyme-inhibitor assay of antitrypsin in serum and the antigen-antibody assay of insulin and its antibody. A signal is displayed directly and is immediately available for chart recording and (or) digital data processing. Since fluorescence polarization offers a number of choices in assay parameters and the use of different fluorescent probes, its development for rapid simultaneous measurements of multiple components in body fluids should be possible.
