Deposition of Lignin Droplets Produced During Dilute Acid Pretreatment of Maize Stems Retards Enzymatic Hydrolysis of Cellulose

Biotechnology Progress - Tập 23 Số 6 - Trang 1333-1339 - 2007
Michael J. Selig1, Sridhar Viamajala1, Stephen R. Decker1, Melvin P. Tucker1, Michael E. Himmel1, Todd B. Vinzant1
1National Renewable Energy Laboratory, 1617 Cole Boulevard, Golden, Colorado, 80401

Tóm tắt

AbstractElectron microscopy of lignocellulosic biomass following high‐temperature pretreatment revealed the presence of spherical formations on the surface of the residual biomass. The hypothesis that these droplet formations are composed of lignins and possible lignin carbohydrate complexes is being explored. Experiments were conducted to better understand the formation of these “lignin” droplets and the possible implications they might have on the enzymatic saccharification of pretreated biomass. It was demonstrated that these droplets are produced from corn stover during pretreatment under neutral and acidic pH at and above 130 °C, and that they can deposit back onto the surface of residual biomass. The deposition of droplets produced under certain pretreatment conditions (acidic pH; T > 150 °C) and captured onto pure cellulose was shown to have a negative effect (5–20%) on the enzymatic saccharification of this substrate. It was noted that droplet density (per unit area) was greater and droplet size more variable under conditions where the greatest impact on enzymatic cellulose conversion was observed. These results indicate that this phenomenon has the potential to adversely affect the efficiency of enzymatic conversion in a lignocellulosic biorefinery.

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Tài liệu tham khảo

10.1016/0273-1177(95)00815-V

10.1007/BF02920531

10.1002/bit.260260110

10.1016/j.biortech.2005.03.040

10.1126/science.1137016

10.1385/ABAB:125:2:077

Donohoe B. S.;Tucker M. P.;Davis M.;Decker S. R.;Himmel M. E.;Vinzant T. B.Tracking lignin coalescence and migration through plant cell walls during pretreatment. In29th Symposium on Biotechnology for Fuels and Chemicals Denver CO 2007; 5B‐01 pP67.

10.1016/S0032-3861(99)00379-1

10.1007/s003960000410

10.1515/hfsg.1995.49.2.182

10.1196/annals.1342.020

10.1016/S0009-2614(01)01022-3

10.1080/10236660490935727

10.1021/jf040456q

Black S. K.;Hames B. R.;Myers M. D.Method of separating lignocellulosic material into lignin cellulose and dissolved sugars. U.S. Patent 5 730 837 1998.

10.1016/j.chroma.2006.02.044

Hatakeyama H., 1992, Methods in Lignin Chemistry, 206

10.1002/bit.20750

10.1016/j.jbiotec.2006.02.021

10.1016/j.jbiotec.2003.09.011

10.1021/jf980407b

10.1038/33653

10.1016/j.carres.2005.09.028

10.1002/bit.20043

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Biotechnology Progress

Tập 23 Số 6

1333-1339

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