Lectin 2 liên kết tế bào hình gai (DCAL2) xác định một phân nhóm tế bào hình gai CD8α− riêng biệt
Tóm tắt
CLRs trên tế bào DC đóng vai trò quan trọng trong miễn dịch và được biểu hiện selektiv trên một số phân nhóm DC nhất định. DCAL2 (lectin C-type ức chế myeloid/Clec12a) ở chuột là một loại CLR kiểu II có ITIM. Sử dụng kháng thể đơn dòng đặc hiệu cho DCAL2 ở chuột, chúng tôi phát hiện rằng DCAL2 được biểu hiện với mức độ tương đối cao trên các APC và rằng biểu hiện DCAL2 có thể được sử dụng để chia CD8α– DCs thành các phân nhóm DCAL2+DCIR2– và DCAL2–DCIR2+. Các phân nhóm CD8α–DCAL2+ DC, CD8α–DCIR2+ DC và CD8α+DCAL2+ DC thể hiện các mức độ TLR khác nhau và phản ứng với các lớp TLR ligand độc đáo bằng cách sản xuất các bộ cytokine khác nhau. Trong khi CD8α–DCAL2+ DCs sản xuất mạnh mẽ cytokine, bao gồm IL-12, khi phản ứng với CpG, CD8α–DCIR2+ DCs chỉ sản xuất TNF-α và IL-10 với số lượng khiêm tốn khi được kích thích bằng zymosan. Tuy nhiên, CD8α–DCIR2+ DCs, không giống như các phân nhóm DC khác, tăng cường OX40L khi được kích thích bằng flagellin vi khuẩn. Theo như dự đoán từ biểu hiện cytokine của chúng, CD8α–DCAL2+ DCs một cách hiệu quả đã kích thích phản ứng Th1 trong sự hiện diện của CpG cả trong ống nghiệm và trong cơ thể sống, trong khi CD8α–DCIR2+ DCs kích thích tế bào Th2 trong đáp ứng với flagellin. Do đó, CD8α–DCAL2+ DCs bao gồm một phân nhóm CD8α– DC đặc biệt có khả năng hỗ trợ các phản ứng Th1. DCAL2 là một dấu hiệu hữu ích để xác định một quần thể tế bào hình gai CD8α– có vai trò kích thích Th1.
Từ khóa
#Dendritic Cell #DCAL2 #Th1 Response #CD8α− Dendritic Cells #CytokinesTài liệu tham khảo
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