Dendritic Cells Fused with Allogeneic Colorectal Cancer Cell Line Present Multiple Colorectal Cancer–Specific Antigens and Induce Antitumor Immunity against Autologous Tumor Cells

Clinical Cancer Research - Tập 11 Số 21 - Trang 7891-7900 - 2005
Shigeo Koido1,2, Eiichi Hara3, Sadamu Homma4, Akihiro Torii1, Yoichi Toyama5, Hidejiro Kawahara5, Michiaki Watanabe5, Katsuhiko Yanaga5, Kiyotaka Fujise1,2, Hisao Tajiri1, Jianlin Gong6, Gotaro Toda1
11Division of Gastroenterology and Hepatology, Department of Internal Medicine;
22Institute of Clinical Medicine and Research;
35Saitama Cancer Center Research Institute for Clinical Oncology, Saitama, Japan; and
43Department of Oncology, Institute of DNA Medicine; and
54Department of Surgery, The Jikei University School of Medicine, Tokyo, Japan;
66Department of Medicine, Boston University School of Medicine, Boston, Massachusetts

Tóm tắt

Abstract The aim of antitumor immunotherapy is to induce CTL responses against autologous tumors. Previous work has shown that fusion of human dendritic cells and autologous tumor cells induce CTL responses against autologous tumor cells in vitro. However, in the clinical setting of patients with colorectal carcinoma, a major difficulty is the preparation of sufficient amounts of autologous tumor cells. In the present study, autologous dendritic cells from patients with colorectal carcinoma were fused to allogeneic colorectal tumor cell line, COLM-6 (HLA-A2−/HLA-24−), carcinoembryonic antigen (CEA)+, and MUC1+ as an alternative strategy to deliver shared colorectal carcinoma antigens to dendritic cells. Stimulation of autologous T cells by the fusion cells generated with autologous dendritic cells (HLA-A2+ and/or HLA-A24+) and allogeneic COLM-6 resulted in MHC class I– and MHC class II–restricted proliferation of CD4+ and CD8+ T cells, high levels of IFN-γ production in both CD4+ and CD8+ T cells, and the simultaneous induction of CEA- and MUC1-specific CTL responses restricted by HLA-A2 and/or HLA-A24. Finally, CTL induced by dendritic cell/allogeneic COLM-6 fusion cells were able to kill autologous colorectal carcinoma by HLA-A2- and/or HLA-A24-restricted mechanisms. The demonstration of CTL activity against shared tumor-associated antigens using an allogeneic tumor cell line, COLM-6, provides that the presence of alloantigens does not prevent the development of CTL with activity against autologous colorectal carcinoma cells. The fusion of allogeneic colorectal carcinoma cell line and autologous dendritic cells could have potential applicability to the field of antitumor immunotherapy through the cross-priming against shared tumor antigens and provides a platform for adoptive immunotherapy.

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Clinical Cancer Research

Tập 11 Số 21

7891-7900

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