Demonstration of Kaposi's Sarcoma-Associated Herpes Virus Cyclin D Homolog in Cutaneous Kaposi's Sarcoma by Colorimetric In Situ Hybridization Using a Catalyzed Signal Amplification System

Blood - Tập 91 Số 10 - Trang 3825-3832 - 1998
Jon A. Reed1,2, Roland G. Nádor1,2, David C. Spaulding1,2, Yoichi Tani1,2, Ethel Cesarman1,2, Daniel M. Knowles1,2
1Dako Corp, Carpinteria, CA.
2From the Department of Pathology, The New York Hospital-Cornell Medical Center, New York, NY; and Dako Corp, Carpinteria, CA.

Tóm tắt

AbstractKaposi's sarcoma-associated herpes virus (KSHV)/human herpes virus 8 (HHV8) DNA sequences have been demonstrated in Kaposi's sarcoma (KS), as well as in some acquired immunodeficiency syndrome (AIDS)-related non-Hodgkin's lymphomas (NHL) and in multicentric Castleman's disease. Although KSHV DNA generally is abundant in KSHV-associated lymphomas, few copies of the virus are present in KS, a property that confounds detection by in situ methods. Previous in situ studies, which identified KSHV in lesions of KS, relied on the use of polymerase chain reaction (PCR) to amplify target DNA sequences before in situ hybridization (ISH) for localization or used ISH with radioactively-labeled probes to obtain adequate levels of detection sensitivity. In this study, a novel nonisotopic nucleic acid ISH method using catalyzed signal amplification and colorimetric detection without PCR-dependent target amplification was used to identify KSHV-specific sequences. The level of sensitivity was increased further by using a probe that detects viral cyclin D homolog transcripts, which are expressed at significant levels during latent viral infection. Thirty cutaneous lesions of KS (25 AIDS-related and five classical European type) were evaluated. AIDS-related NHL and cell lines derived from patients with AIDS-related NHL, all of which were known to harbor KSHV by Southern blot analysis, were used as positive controls. NHL and benign cutaneous vascular lesions not associated with AIDS were used as negative controls. For each of the 30 KS lesions studied, hybridization signals were detected in most of the spindle cells surrounding the atypical slit-like vascular channels and also were detected in some endothelial cells in well-formed blood vessels in the perilesional dermis. Plaque and nodular lesions generally contained more labeled cells than did early patch lesions. All AIDS-related NHL and cell lines contained KSHV-specific sequences; however, the non-AIDS–related NHLs and benign vascular lesions were negative. These results confirm the presence of KSHV sequences in cutaneous KS and provide in situ evidence of infection by this virus in early patch-stage lesions. This study also defines the in situ expression of the KSHV cyclin D homolog viral oncogene in cutaneous KS. The use of this sensitive nonisotopic ISH method should allow detection of other KSHV-specific gene products, further defining the pathobiology of this virus.

Từ khóa


Tài liệu tham khảo

Chang, 1994, Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma., Science, 266, 1865, 10.1126/science.7997879

Moore, 1996, Primary characterization of a herpesvirus agent associated with Kaposi's sarcoma., J Virol, 70, 549, 10.1128/JVI.70.1.549-558.1996

Said, 1996, Kaposi's sarcoma-associated herpesvirus (KSHV or HHV8) in primary effusion lymphoma: Ultrastructural demonstration of herpesvirus in lymphoma cells., Blood, 87, 4937, 10.1182/blood.V87.12.4937.bloodjournal87124937

Cesarman, 1995, Kaposi's sarcoma-associated herpesvirus-like DNA sequences in AIDS-related body-cavity-based lymphomas., N Engl J Med, 332, 1186, 10.1056/NEJM199505043321802

Nador, 1996, Primary effusion lymphoma: A distinct clinicopathologic entity associated with the Kaposi's sarcoma-associated herpes virus., Blood, 88, 645, 10.1182/blood.V88.2.645.bloodjournal882645

Soulier, 1995, Kaposi's sarcoma-associated herpesvirus-like DNA sequences in multicentric Castleman's disease., Blood, 86, 1276, 10.1182/blood.V86.4.1276.bloodjournal8641276

Jin, 1996, Detection of Kaposi's sarcoma-associated herpesvirus-like DNA sequences in vascular lesions. A reliable marker for Kaposi's sarcoma., Am J Clin Pathol, 105, 360, 10.1093/ajcp/105.3.360

Dictor, 1996, Human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus) DNA in Kaposi's sarcoma lesions, Kaposi's sarcoma cell lines, endothelial Kaposi's sarcoma simulators, and the skin of immunosuppressed patients., Am J Pathol, 148, 2009

Moore, 1995, Detection of herpesvirus-like DNA sequences in Kaposi's sarcoma in patients with and without HIV infection., N Engl J Med, 332, 1181, 10.1056/NEJM199505043321801

Buonaguro, 1996, Herpesvirus-like DNA sequences detected in endemic, classic, iatrogenic and epidemic Kaposi's sarcoma (KS) biopsies., Int J Cancer, 65, 25, 10.1002/(SICI)1097-0215(19960103)65:1<25::AID-IJC5>3.0.CO;2-3

Chuck, 1996, Frequent presence of a novel herpesvirus genome in lesions of human immunodeficiency virus-negative Kaposi's sarcoma., J Infect Dis, 173, 248, 10.1093/infdis/173.1.248

de Lellis, 1995, Herpesvirus-like DNA sequences in non-AIDS Kaposi's sarcoma., J Infect Dis, 172, 1605, 10.1093/infdis/172.6.1605

Noel, 1996, Herpesvirus-like DNA sequences and Kaposi's sarcoma: Relationship with epidemiology, clinical spectrum, and histologic features., Cancer, 77, 2132, 10.1002/(SICI)1097-0142(19960515)77:10<2132::AID-CNCR26>3.0.CO;2-V

Boshoff, 1995, Kaposi's sarcoma-associated herpesvirus infects endothelial and spindle cells., Nat Med, 1, 1274, 10.1038/nm1295-1274

Huang, 1996, Transcription of human herpesvirus-like agent (HHV-8) in Kaposi's sarcoma., J Clin Invest, 97, 2803, 10.1172/JCI118735

Li, 1996, Localization of human herpes-like virus type 8 in vascular endothelial cells and perivascular spindle-shaped cells of Kaposi's Sarcoma lesions by in situ hybridization., Am J Pathol, 148, 1741

Staskus, 1997, Kaposi's sarcoma-associated herpesvirus gene expression in endothelial (spindle) tumor cells., J Virol, 71, 715, 10.1128/JVI.71.1.715-719.1997

Bobrow, 1989, Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays., J Immunol Methods, 125, 279, 10.1016/0022-1759(89)90104-X

Bobrow, 1991, Catalyzed reporter deposition, a novel method of signal amplification. II. Application to membrane immunoassays., J Immunol Methods, 137, 103, 10.1016/0022-1759(91)90399-Z

Raap, 1995, Ultra-sensitive FISH using a peroxidase-mediated deposition of biotin- or fluorichrome tyramides., Hum Mol Genet, 4, 529, 10.1093/hmg/4.4.529

Zehbe, 1997, Sensitive in situ hybridization with catalyzed reporter deposition, streptavidin-nanogold, and silver acetate autometallography. Detection of single-copy human papillomavirus., Am J Pathol, 150, 1553

Decker, 1996, The Kaposi sarcoma-associated herpesvirus (KSHV) is present as an intact latent genome in KS tissue but replicates in the peripheral blood mononuclear cells of KS patients., J Exp Med, 184, 283, 10.1084/jem.184.1.283

Zhong, 1996, Restricted expression of Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) genes in Kaposi's sarcoma., Proc Natl Acad Sci USA, 93, 6641, 10.1073/pnas.93.13.6641

Cesarman, 1996, Kaposi's sarcoma-associated herpesvirus contains G protein-coupled receptor and cyclin D homologs which are expressed in Kaposi's sarcoma and malignant lymphoma., J Virol, 70, 8218, 10.1128/JVI.70.11.8218-8223.1996

Russo, 1996, Nucleotide sequence of the Kaposi's sarcoma-associated herpesvirus (HHV8)., Proc Natl Acad Sci USA, 93, 14862, 10.1073/pnas.93.25.14862

Chang, 1996, Cyclin encoded by KS herpesvirus., Nature, 382, 410, 10.1038/382410a0

Peters, 1994, The D-type cyclins and their role in tumorigenesis., J Cell Sci, 18, 89, 10.1242/jcs.1994.Supplement_18.13

Arnold, 1989, Molecular cloning and chromosomal mapping of DNA rearranged with the parathyroid hormone gene in a parathyroid adenoma., J Clin Invest, 83, 2034, 10.1172/JCI114114

Motokura, 1991, A novel cyclin encoded by a bcl1-linked candidate oncogene., Nature, 350, 512, 10.1038/350512a0

Zhang, 1993, Amplification and oversexpression of cyclin D1 in human hepatocellular carcinoma., Biochem Biophys Res Commun, 196, 1010, 10.1006/bbrc.1993.2350

Tsujimoto, 1984, Cloning of the chromosome breakpoint of neoplastic B cells with the t(14;18) chromosome translocation., Science, 226, 1097, 10.1126/science.6093263

Cesarman, 1995, In vitro establishment and characterization of two acquired immunodeficiency syndrome-related lymphoma cell lines (BC-1 and BC-2) containing Kaposi's sarcoma-associated herpesvirus-like (KSHV) DNA sequences., Blood, 86, 2708, 10.1182/blood.V86.7.2708.bloodjournal8672708

Flore, 1997, Effect of DNA synthesis inhibitors on KSHV cyclin and major capsid protein (MCP) gene expression., AIDS Res Hum Retroviruses, 13, 1229, 10.1089/aid.1997.13.1229

Li, 1997, Kaposi's sarcoma-associated herpesvirus encodes a functional cyclin., J Virol, 71, 1984, 10.1128/JVI.71.3.1984-1991.1997

Arvanitakis, 1996, Establishment and characterization of a body cavity-based lymphoma cell line (BC-3) harboring Kaposi's sarcoma-associated herpesvirus in the absence of EBV., Blood, 88, 2648, 10.1182/blood.V88.7.2648.bloodjournal8872648

Miller, 1988, A simple salting out procedure for extracting DNA from human nucleated cells., Nucleic Acids Res, 16, 1215, 10.1093/nar/16.3.1215

Southern, 1975, Detection of specific sequences among DNA fragments separated by gel electrophoresis., J Mol Biol, 98, 503, 10.1016/S0022-2836(75)80083-0

Pelicci, 1985, Lymphoid tumors displaying rearrangements of both immunoglobulin and T cell receptor genes., J Exp Med, 162, 1015, 10.1084/jem.162.3.1015

Iezzoni, 1992, Colorimetric detection of herpes simplex virus by DNA in situ sandwich hybridization: A rapid, formamide-free, random oligomer-enhanced method., Nucleic Acids Res, 20, 1149, 10.1093/nar/20.5.1149

Reed, 1992, Complete one-hour immunocytochemistry based on capillary action., Biotechniques, 13, 434

Archibald, 1992, Evidence for a sexually transmitted cofactor for AIDS-related Kaposi's sarcoma in a cohort of homosexual men., Epidemiology, 3, 203, 10.1097/00001648-199205000-00004

Jaffe, 1983, National case-control study of Kaposi's sarcoma and Pneumocystis carinii pneumonia in homosexual men: Part I. Epidemiologic results., Ann Intern Med, 99, 145, 10.7326/0003-4819-99-2-145

Lassoued, 1991, AIDS-associated Kaposi's sarcoma in female patients., AIDS, 5, 877, 10.1097/00002030-199107000-00013

Renne, 1996, Lytic growth of Kaposi's sarcoma-associated herpesvirus (human herpes virus 8) in culture., Nat Med, 2, 342, 10.1038/nm0396-342

Mesri, 1996, Human herpesvirus-8/Kaposi's sarcoma-associated herpesvirus is a new transmissible virus that infects B cells., J Exp Med, 183, 2385, 10.1084/jem.183.5.2385

Whitby, 1995, Detection of Kaposi's sarcoma associated herpesvirus in peripheral blood of HIV-infected individuals and progression to Kaposi's sarcoma., Lancet, 346, 799, 10.1016/S0140-6736(95)91619-9

Gao, 1996, Seroconversion to antibodies against Kaposi's sarcoma-associated herpesvirus-related latent nuclear antigens before the development of Kaposi's sarcoma., N Engl J Med, 335, 233, 10.1056/NEJM199607253350403

Thông tin
Thông tin xuất bản

Blood

Tập 91 Số 10

3825-3832

Thông tin tác giả