Philippe Chadebech1, Anoosha Habibi1, Ruben Nzouakou1, Dora Bachir1, Natacha Meunier‐Costes1, Patricia Bonin1, M. Rodet1, Btissam Chami1, Frédéric Galactéros1, Philippe Bierling1, F. Noizat‐Pirenne1
1From the Etablissement Français du Sang, Ile de France, Hôpital Henri Mondor, and the Unité des Maladies Génétiques du Globule Rouge, Hôpital Henri Mondor, Créteil, France.
Tóm tắt
BACKGROUND: Delayed hemolytic transfusion reaction (DHTR) is a life‐threatening complication in sickle cell disease (SCD) characterized by recurrence of disease complications, recipient red blood cell (RBC) destruction, and frequently no detectable antibody. Phosphatidylserine (PS) exposure signs suicidal RBC death or eryptosis and is involved in vasoocclusive crisis (VOC).STUDY DESIGN AND METHODS: Transfusion was monitored in 48 SCD patients for up to 20 days. PS exposure was evaluated in vivo on patient RBCs (PS‐RBCs) at five time points and in vitro after incubation of donor RBCs with pretransfusion plasma.RESULTS: Three VOC patients displayed DHTR with recurrent SCD features and no detectable antibody in two cases. In vitro, PS‐RBC percentage was significantly increased by incubating donor RBCs with pretransfusion plasma samples from DHTR patients with no detectable antibody. No such increase was observed with samples from other patients. This result indicates that donor RBCs may be damaged by the environment of SCD patients, increasing the physiologic clearance of apoptotic RBCs. In vivo, PS‐RBC percentage increased in all three cases after destruction of transfused RBCs, indicating that DHTR induces PS‐RBCs and, possibly, subsequent VOC and autologous RBC destruction.CONCLUSION: This study clearly demonstrates that DHTR can occur in the absence of detectable antibody. In these cases, a mechanism of excessive eryptosis is proposed.
