Nanuli Doreulee1, Ritchie E. Brown1, Yevgenij Yanovsky1, Axel Gödecke2, Jürgen Schrader2, Helmut L. Haas1
1Institut für Neurophysiologie, Heinrich-Heine-Universität, D-40001 Düsseldorf, Germany
2Institut für Herz- und Kreislaufphysiologie, Heinrich-Heine-Universität, D-40001 Düsseldorf, Germany
Tóm tắt
AbstractMossy fiber long‐term potentiation (mfLTP) was compared in hippocampal slices prepared from wild‐type mice and mice lacking functional endothelial nitric oxide synthase (eNOS‐/‐ mice) using field potential recording. In the presence of D‐2‐amino‐5‐phosphonovaleric acid (AP5, 50 μM), the mfLTP induced by tetanic stimulation (100 Hz, 1 sec) was significantly reduced in knockouts (n = 8) in comparison with wild‐type (n = 8). Similarly, potentiation induced by forskolin (30 μM) or 8‐bromo‐cyclic adenosine monophosphate (8‐Br‐cAMP, 100 μM) was less pronounced in knockouts. However, in wild‐types the mfLTP‐induced in the presence of the nonselective pharmacological inhibitor of NOS (N‐nitro‐L‐Arginine, 100 μM, n = 6) was not significantly different from control (n = 8). Thus, eNOS is not directly involved in mfLTP, but lack of eNOS during development leads to a deficit downstream of adenylyl cyclase. Synapse 41:191–194, 2001. © 2001 Wiley‐Liss, Inc.
