Decreased paraoxonase 1 activity and increased oxidative stress in low lead−exposed workers

Human and Experimental Toxicology - Tập 30 Số 9 - Trang 1196-1203 - 2011
Thinnakorn Permpongpaiboon1,2, Amar Nagila2,3, Phannee Pidetcha4, Kulwadee Tuangmungsakulchai2, Soontharee Tantrarongroj2, Sureerut Porntadavity2
1Department of Clinical Chemistry, Faculty of Medical Technology, Huachiew Chalermprakiet, Samut Prakarn, Thailand
2Department of Clinical Chemistry, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand
3Fishtail Hospital and Research Center, Pokhara Gandaki Zone, Nepal
4Central of Medical Service, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand

Tóm tắt

Paraoxonase 1 (PON1) has been proposed as an antioxidant enzyme. Although lead-inhibited PON1 activity has been demonstrated mostly based on in vitro experiments, it is uncertain whether this phenomenon is relevant in pathogenesis of lead-induced oxidative stress in the lead exposure. We examined associations of blood lead levels (BLL) and PON1 activity along with oxidative stress parameters in lead exposure workers. We determined malondialdehyde (MDA), conjugated diene (CD), total peroxides (TP), total antioxidant status (TAS), the oxidative stress index (OSI), and PON1 activity in earthenware factory workers ( n = 60) and control subjects ( n = 65). The lead-exposed group significantly increased lipid peroxidation parameters and OSI compared to the control group ( p < 0.001). The lead-exposed group had significantly decreased PON1 activity and TAS levels compared to the control group ( p < 0.001). Multiple linear regression analysis revealed that BLL were significantly correlated with decreased TAS ( r = −0.496) and PON1 activity ( r = −0.434), but with increased CD ( r = 0.694), TP ( r = 0.614), MDA ( r = 0.788), and OSI ( r = 0.722). Interestingly, BLL at 10 µg/dL significantly decreased PON1 activity and increased oxidative stress parameters with insignificant changes in other biochemical and hematological parameters. Altogether, the reduction of PON1 activity may associate in an imbalance in pro-oxidants and antioxidants, leading to oxidative damage in lead-exposed workers even at low BLL.

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Tài liệu tham khảo

International Agency for Research on Cancer (IARC). Monographs on the evaluation of the carcinogenic risk to human: Inorganic and organic lead compounds. Lyon 2006, p.87.

10.1201/9780824751999.ch10

10.1007/BF01973308

10.1161/01.CIR.0000130848.18636.B2

10.1016/j.tox.2005.05.003

10.1001/archinte.162.21.2443

10.1161/CIRCULATIONAHA.106.628321

10.2105/AJPH.89.7.1083

10.1016/S0895-7061(99)00226-5

10.1016/j.cca.2005.12.024

10.1016/j.envres.2004.05.016

10.1016/j.tox.2003.09.009

10.1016/0014-5793(91)80962-3

10.1016/0021-9150(93)90183-U

10.1016/0006-2952(94)90200-3

10.1016/S0009-2797(97)00046-X

10.1016/S0162-0134(02)00627-X

10.1016/j.cbi.2007.01.006

Cole TB, 2000, Toxicol Sci, 66, 312

10.1289/ehp.9163

10.1016/S0076-6879(78)52032-6

10.1016/j.ijcard.2004.05.069

10.1093/clinchem/43.7.1209

Eckerson HW, 1983, Am J Hum Genet, 35, 1126

10.1146/annurev.med.55.091902.103653

Khalil-Manesh F, 1994, J Trace Elem Electrolytes Health Dis, 8, 13

10.1177/174182679400100114

10.1038/sj.jhh.1001300

10.1016/S0002-9149(98)00402-0

10.1016/j.amjcard.2006.05.056

10.1097/00041552-199405000-00005

10.1161/01.HYP.30.6.1487

Kasperczyk S, 2005, Ann Agric Environ Med, 12, 207

10.1016/j.cca.2007.04.024

10.1021/ja00537a032

Sugawara E, 1991, Br J Ind Med, 48, 239

10.1289/ehp.8518

Occupational Safety and Health Administration (OSHA). Occupational Safety and Health Standards: Toxic and Hazardous Substances: Lead 29CFR1910.1025.

10.2478/10004-1254-59-2008-1883

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Human and Experimental Toxicology

Tập 30 Số 9

1196-1203

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