DOG1 and CD117 are the antibodies of choice in the diagnosis of gastrointestinal stromal tumours

Histopathology - Tập 57 Số 2 - Trang 259-270 - 2010
Marco Novelli1,2, Sabrina Rossi3,2, Manuel Rodriguez‐Justo1, Philippe Tanière4, Beatrice Seddon5, Luisa Toffolatti3, Chiara Sartor3, Pancras C.W. Hogendoorn6, Raf Sciot7, M. van Glabbeke8, Jaap Verweij9, Jean‐Yves Blay10, Peter Hohenberger11, Adrienne M. Flanagan1, Angelo Paolo Dei Tos3
1Department of Pathology, University College London NHS Trust, London, UK
2These authors contributed equally to this manuscript
3Department of Pathology, General Hospital of Treviso, Italy
4Histopathology, University Hospital Birmingham NHS Foundation Trust, Birmingham
5Department of Oncology, University College London NHS Trust, London, UK
6Department of Pathology, Leiden Medical Center, the Netherlands
7Department of Pathology, University of Leuven, Leuven
8EORTC Data Center, Brussels, Belgium
9Department of Medical Oncology, Erasmus University Medical Center, Rotterdam, The Netherlands
10Department of medical oncology, Centre Leon Berard, Lyon, France
11Division of Surgical Oncology and Thoracic Surgery, Mannheim University Medical Center, Mannheim, Germany

Tóm tắt

Novelli M, Rossi S, Rodriguez‐Justo M, Taniere P, Seddon B, Toffolatti L, Sartor C, Hogendoorn P C W, Sciot R, Van Glabbeke M, Verweij J, Blay J Y, Hohenberger P, Flanagan A & Dei Tos A P
(2010) Histopathology57, 259–270
DOG1 and CD117 are the antibodies of choice in the diagnosis of gastrointestinal stromal tumoursAims:  The histopathological diagnosis of gastrointestinal stromal tumours (GIST) is typically made based on a combination of clinical and morphological features supported by immunohistochemistry studies. The aim of this study was to examine the staining quality, sensitivity, specificity and utility of antibodies used commonly in GIST diagnosis.Methods and results:  Immunohistochemistry with a panel of antibodies [CD117, DOG1, protein kinase C (PKC)‐theta, nestin, CD34, smooth muscle actin (SMA), desmin, S100 and CD171] was performed on whole sections from 187 GIST and 29 gastrointestinal mesenchymal tumours, and on several microarrays including 355 GISTs and 120 soft tissue sarcomas. Results showed that DOG1 and CD117 were the most sensitive and specific antibodies used in GIST diagnosis. PKC‐theta and nestin were sensitive, but less specific, also staining other spindle cell tumours commonly considered in the differential diagnosis of GIST. CD34 staining was less sensitive than many of the other antibodies and of limited aid in diagnosis. The smooth muscle markers SMA and desmin, together with the neural marker S100, were unhelpful in confirming a diagnosis of GIST, but were particularly useful in the exclusion/diagnosis of other gastrointestinal mesenchymal tumour types.Conclusions:  In the majority of histologically suspected GISTs a combination of CD117 and DOG1 immunostaining is sufficient to confirm the histological diagnosis.

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Histopathology

Tập 57 Số 2

259-270

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