DNA damage in Pakistani agricultural workers exposed to mixture of pesticides

Environmental and Molecular Mutagenesis - Tập 50 Số 1 - Trang 37-45 - 2009
Javed A. Bhalli1, Tayyaba Ali1, Muhammad Rafiq Asi2, Zafar M. Khalid1, Marcello Ceppi3, Qaiser M. Khan4
1Environmental Toxicology Laboratory, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan
2Pesticides Chemistry Laboratory, Nuclear Institute of Agriculture and Biology (NIAB), Faisalabad, Pakistan
3Unit of Molecular Epidemiology, National Cancer Research Institute of Genoa, Italy#TAB#
4Environmental Toxicology Laboratory, National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box No. 577, Jhang Road, Faisalabad, Pakistan

Tóm tắt

AbstractA cross‐sectional study was designed to determine whether occupational exposure to a complex mixture of pesticides results in a significant increase of DNA damage in farmers chronically exposed to pesticides in open fields. Leukocytes from 47 agriculture workers exposed to pesticides and 50 controls were evaluated with comet assay. Workers recruitment was based on their exposure to pesticides during the spraying season on cotton crop. Serum from these individuals was also analyzed for pesticides presence using high performance liquid chromatography. Statistically significant difference (P < 0.001) in DNA damage of exposed individuals (mean ± S.D 14.80 ± 3.04 μm) was observed when compared with control group (6.54 ± 1.73 μm) as studied on the basis of comet tail length. Smokers had significantly higher mean comet tail length than nonsmokers and ex‐smokers in both workers (20.26 ± 3.53 vs. 14.19 ± 4.25, P < 0.001) and controls (7.86 ± 1.09 vs. 5.80 ± 1.59, P < 0.001), whereas age had a minimal effect on DNA damage (P < 0.05). The length of pesticide exposure is positively associated with DNA damage in exposed individuals (P < 0.001). Our study shows that chronic exposure to pesticides produces DNA damage in pesticide sprayers and suggests that this type of monitoring is recommended in preventive policies for pesticide sprayers. Environ. Mol. Mutagen., 2009. © 2008 Wiley‐Liss, Inc.

Từ khóa


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Environmental and Molecular Mutagenesis

Tập 50 Số 1

37-45

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