DNA barcoding Indian marine fishes

Molecular Ecology Resources - Tập 11 Số 1 - Trang 60-71 - 2011
W. S. Lakra1, Mukti Verma1, Mukunda Goswami1, J. K. Jena1, Vindhya Mohindra1, Peyush Punia1, A. Gopalakrishnan1, Karan Veer Singh1, Robert Ward2, Paul D. N. Hebert3
1National Bureau of Fish Genetic Resources, Lucknow‐226002, India
2CSIRO Marine and Atmospheric Research, GPO Box 1538, Hobart, Tasmania 7001, Australia
3Biodiversity Institute of Ontario, University of Guelph, Ontario, Canada N1G 2WI

Tóm tắt

AbstractDNA barcoding has been adopted as a global bio‐identification system for animals in recent years. A major national programme on DNA barcoding of fish and marine life was initiated in India by the authors during 2006 and 115 species of marine fish covering Carangids, Clupeids, Scombrids, Groupers, Sciaenids, Silverbellies, Mullids, Polynemids and Silurids representing 79 Genera and 37 Families from the Indian Ocean have been barcoded for the first time using cytochrome c oxidase I gene (COI) of the mtDNA. The species were represented by multiple specimens and a total of 397 sequences were generated. After amplification and sequencing of 707 base pair fragment of COI, primers were trimmed which invariably generated a 655 base pair barcode sequence. The average Kimura two parameter (K2P) distances within species, genera, families, orders were 0.30%, 6.60%, 9.91%, 16.00%, respectively. In addition to barcode‐based species identification system, phylogenetic relationships among the species have also been attempted. The neighbour‐joining tree revealed distinct clusters in concurrence with the taxonomic status of the species.

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Molecular Ecology Resources

Tập 11 Số 1

60-71

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