Cytoplasmic male sterility in sunflower is correlated with the co-transcription of a new open reading frame with the atpA gene
Tóm tắt
The organization and expression of the mitochondrial (mt) genome of fertile, male-sterile and restored lines of Helianthus annuus and of H. petiolaris were compared to identify alterations which might lead to cytoplasmic male sterility (CMS). The mtDNAs of fertile and male-sterile lines differ by an 11 kb inversion and a 5 kb insertion. The rearrangements seem to be the result of recombination events within an inverted repeat of 261 bp. Detectable alterations in the transcript pattern of the rearranged mtDNA regions are restricted to the atpA locus. The male-sterile line CMSBaso shows three additional transcripts of the atpA locus of about 2500, 1200 and 250 nucleotides which are not detectable in Baso. However, the coding sequences of the atpA gene are entirely identical in the fertile line Baso and the male-sterile line CMSBaso. But a new open reading frame (orfH522) of 522 nucleotides is co-transcribed with the atpA gene as an additional larger transcript of about 2500 nucleotides in CMSBaso. orfH522 is also included in a second additional transcript of about 1200 nucleotides. The predicted translation product of orfH522 might play a role in CMS in sunflower.
Tài liệu tham khảo
Bailey-Serres JD, Hanson DK, Fox TD, Leaver CJ (1986) Mitochondrial genome rearrangement leads to extension and relocation of the Cytochrome c oxidase subunit I gene in sorghum. Cell 47:567–576
Bland MM, Levings CS III, Matzinger DF (1986) The tobacco mitochondrial ATPase subunit 9 gene is closely linked to an open reading frame for a ribosomal protein. Mol Gen Genet 204:8–16
Clark EM, Izhar S, Hanson MR (1985) Independent segregation of the plastid genome and cytoplasmic male sterility in Petunia somatic hybrids. Mol Gen Genet 199:440–445
Covello PS, Gray MW (1989) RNA editing in plant mitochondria. Nature 341:662–666
Crouzillat D, Leroy P, Perrault A, Ledoigt G (1987) Molecular analysis of the mitochondrial genome of Helianthus annuus in relation to cytoplasmic male sterility and phylogeny. Theor Appl Genet 74:773–780
Dewey RE, Levings CS III, Timothy DH (1986) Novel recombinations in maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm. Cell 44:439–449
Dewey RE, Siedow JN, Timothy DH, Levings CS III (1988) A 13 kilodalton maize mitochondrial protein in E. coli confers sensitivity to Bipolaris maydis toxin. Science 239:293–295
Finnigan PM, Brown GG (1990) Transcriptional and post-transcriptional regulation of RNA levels in maize mitochondria. Plant Cell 2:71–83
Gualberto JM, Lamattina L, Bonnard G, Weil J-C, Grienenberger J-M (1989) RNA editing in wheat mitochondria results in the conservation of protein sequences. Nature 341:660–662
Hanson MR, Conde MF (1985) Functioning and variation of cytoplasmic genomes: lessons from cytoplasmic-nuclear interactions affecting male fertility in plants. Int Rev Cytol 94:213–267
Herdenberger F, Evrard JL, Kuntz M, Tessier LH, Klein A, Steinmetz A, Pillay DTN (1990) Isolation of flower-specific cDNA clones from sunflower. Plant Sci 69:111–122
Hiesel R, Brennicke A (1985) Overlapping reading frames in Oenothera mitochondria. FEBS Lett 193:164–168
Hiesel R, Schobel W, Schuster W, Brennicke A (1987) The cytochrome oxidase subunit I and subunit III genes in Oenothera mitochondria are transcribed from identical promotor sequences. EMBO J 6:29–34
Horn R, Köhler RH, Zetsche K (1991) A mitochondrial 16 kDa protein is associated with cytoplasmic male sterility in sunflower. Plant Mol Biol, in press
Köhler RK, Lössl A, Zetsche K (1990) Nucleotide sequence of the F1-ATPase subunit α gene of sunflower mitochondria. Nucleic Acids Res 18:4588
Kröger M, Kröger-Block A (1984) Extension of a flexible computer program for handling DNA sequence data. Nucleic Acids Res 12:113–123
Leaver CJ, Gray MW (1982) Mitochondrial genome organization and expression in higher plants. Annu Rev Plant Physiol 33:373–402
Leclercq P (1969) Une stérilité mâle cytoplasmique chez le tournesol. Ann Amelior Plantes 19:99–106
Leroy P, Bazetoux S, Quétier F, Delbut J, Berville A (1985) A comparison between mitochondrial DNA of an isogenic malesterile (S) and male-fertile (F) couple (HA89) of sunflower. Curr Genet 9:245–251
Makaroff CA, Apel IJ, Palmer JD (1989) The atp6 coding region has been disrupted and a novel reading frame generated in the mitochondrial genome of cytoplasmic male-sterile radish. J Biol Chem 264:11706–11713
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473–497
Nivison HT, Hanson MH (1989) Identification of a mitochondrial protein associated with cytoplasmic male sterility in Petunia. Plant Cell 1:1121–1130
Pruitt KD, Hanson MR (1989) Cytochrome oxidase subunit II sequences in Petunia mitochondria: two intron-containing genes and an intron-less pseudogene associated with cytoplasmic male sterility. Curr Genet 16:281–291
Quagliariello C, Saiardi A, Gallerani R (1990) The cytochrome oxidase subunit III gene in sunflower mitochondria is cotranscribed with an open reading frame conserved in higher plants. Curr Genet 18:355–363
Rasmussen J, Hanson MR (1989) A NADH dehydrogenase subunit gene is co-transcribed with the abnormal Petunia mitochondrial gene associated with cytoplasmic male sterility. Mol Gen Genet 215:332–336
Recipon H (1990) The sequence of the sunflower mitochondrial ATPase subunit 9 gene. Nucleic Acids Res 18:1644
Rogers SO, Bendich AJ (1988) Extraction of DNA from plant tissues. Plant Mol Biol Manual A6:1–10
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Schuster W, Brennicke A (1985) TAG-Termination codon in the apocytochrome b gene from Oenothera mitochondria. Curr Genet 9:157–163
Schuster W, Brennicke A (1986) Pseudocopies of the ATPase α-subunit gene in Oenothera mitochondria are present on different circular molecules. Mol Gen Genet 204:29–35
Schuster W, Hiesel R, Isaac PG, Leaver CJ, Brennicke A (1986) Transcript termini of messenger RNAs in higher plant mitochondria. Nucleic Acids Res 14:5943–5954
Siculella L, Palmer JD (1988) Physical and gene organization of mitochondrial DNA in fertile and male-sterile sunflower. CMS-associated alterations in structure and transcription of the atpA gene. Nucleic Acids Res 16:3787–3799
Siculella L, D'Ambrosio L, De Tuglie AD, Gallerani R (1990) Minor differences in the primary structure of the atpA genes coded on the mtDNA of fertile and male-sterile sunflower lines. Nucleic Acids Res 18:4599
Stern DB, Newton KJ (1986) Isolation of plant mitochondrial RNA. Methods Enzymol 118:488–496
Taylor B, Powell A (1982) Isolation of Plant DNA and RNA. Bethesda Research Laboratories, Focus 4:3;4–6
Wise PR, Pring DR, Gegenbach BG (1987) Mutation to male fertility and toxin insensitivity in Texas (T)-cytoplasm maize is associated with a frameshift in a mitochondrial open reading frame. Proc Natl Acad Sci USA 84:2858–2862
Wissinger B, Hiesel R, Schuster W, Brennicke A (1988) The NADH-dehydrogenase subunit 5 gene in Oenothera mitochondria contains two introns and is co-transcribed with the 5S rRNA gene. Mol Gen Gen 212:56–65
Young EG, Hanson MR (1987) A fused mitochondrial gene associated with cytoplasmic male sterility is developmentally regulated. Cell 50:41–49