Cytokinin modulates proteomic, transcriptomic and growth responses to temperature shocks in <scp><i>A</i></scp><i>rabidopsis</i>

Plant, Cell and Environment - Tập 37 Số 7 - Trang 1641-1655 - 2014
Martin Černý1, Petr Jedelský2, Jan Novák1, Andreas Schlösser3, Břetislav Brzobohatý1
1Laboratory of Plant Molecular Biology, Institute of Biophysics AS CR, v.v.i and CEITEC – Central European Institute of Technology Mendel University in Brno CZ‐613 00 Brno Czech Republic
2Department of Cell Biology Faculty of Science Charles University CZ‐128 43 Prague Czech Republic
3Core Facility Proteomics, ZBSA University of Freiburg 79104 Freiburg Germany

Tóm tắt

AbstractAs sessile organisms, plants must sense environmental conditions and adjust their growth and development processes accordingly, through adaptive responses regulated by various internal factors, including hormones. A key environmental factor is temperature, but temperature‐sensing mechanisms are not fully understood despite intense research. We investigated proteomic responses to temperature shocks (15 min cold or heat treatments) with and without exogenous applications of cytokinin in Arabidopsis. Image and mass spectrometric analysis of the two‐dimensionally separated proteins detected 139 differentially regulated spots, in which 148 proteins were identified, most of which have not been previously linked to temperature perception. More than 70% of the temperature‐shock response proteins were modulated by cytokinin, mostly in a similar manner as heat shock. Data mining of previous transcriptomic datasets supported extensive interactions between temperature and cytokinin signalling. The biological significance of this finding was tested by assaying an independent growth response of Arabidopsis seedlings to heat stress: hypocotyl elongation. This response was strongly inhibited in mutants with deficiencies in cytokinin signalling or endogenous cytokinin levels. Thus, cytokinins may directly participate in heat signalling in plants. Finally, large proportions of both temperature‐shock and cytokinin responsive proteomes co‐localize to the chloroplast, which might therefore host a substantial proportion of the temperature response machinery.

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Plant, Cell and Environment

Tập 37 Số 7

1641-1655

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