Cytogenetic analyses of mice exposed to dichloromethane

Environmental and Molecular Mutagenesis - Tập 15 Số 4 - Trang 221-228 - 1990
James W. Allen1, Andrew D. Kligerman2, James A. Campbell3, Barbara Westbrook‐Collins2, Gregory L. Erexson3, Frank W. Kari4, Errol Zeiger5
1Genetic Toxicology Division, U.S. Environmental Protection Agency, Research Research Triangle Park, NC 27711.
2Genetic Toxicology Division (MD‐68), Health Effects Research Laboratory, U.S. Environmental Protection Agency, North Carolina
3Environmental Health Research and Testing, Inc., North Carolina
4Toxicology Evaluation Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina
5Cellular and Genetic Toxicology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina

Tóm tắt

AbstractChromosome damage was studied in female B6C3F1 mice exposed to dichloromethane (DCM) by subcutaneous or inhalation treatments. No increase in the frequency of either sister chromatid exchanges (SCEs) or chromosome aberrations (CAs) in bone marrow cells was observed after a single subcutaneous injection of 2,500 or 5,000 mg/kg DCM. Inhalation exposure to DCM for 10 days at concentrations of 4,000 or 8,000 ppm resulted in significant increases in frequencies of SCEs in lung cells and peripheral blood lymphocytes, CAs in lung and bone marrow cells, and micronuclei (MN) in peripheral blood erythrocytes. Lung cell CAs and blood erythrocyte MN reached frequencies of approximately two times control levels. Following a 3‐month inhalation exposure to 2,000 ppm DCM, mice showed small but significant increases in lung cell SCEs and peripheral blood erythrocyte MN. These findings suggest that genotoxicity may play a role in the carcinogenicity of DCM in the lungs of B6C3F1 female mice.

Từ khóa


Tài liệu tham khảo

10.1007/BF01538862

Anderson BE EzeigerMDShelby MAResnick DKGulatiJLandKSLoveaay(1989):Chromosome aberration and sister chromatid exchange test results with 42 chemicals Environ Mol Mutagen in press.

10.1016/0272-0590(84)90217-3

Campbell JA, 1986, Sister chromatid exchange analysis in cultured primary lung, liver, and kidney cells of mice following in vivo exposure to vinyl carbamate, In Vitro, 22, 443

10.1016/B978-0-12-336180-6.50014-4

10.1080/01621459.1955.10501294

10.1002/em.2850100407

10.1016/0027-5107(87)90093-5

10.1016/0027-5107(86)90010-2

10.1007/BF00328535

10.1016/0165-1218(83)90211-2

Heck H d'A, 1989, Toxicologic implications of formaldehyde formation in vivo from industrial chemicals and pharmaceuticals, CIIT Activities, 9, 1

International Agency for Research on Cancer(1979):IARC monographs on the evaluation of the carcinogenic risk of chemicals to humans. Some halogenated hydrocarbons vol. 20 pp449–465.

International Agency for Research on Cancer(1986):IARC monographs on the evaluation of the carcinogenic risk of chemicals to humans. Some halogenated hydrocarbons and pesticide exposures vol. 41 pp43–85.

10.1016/0027-5107(78)90191-4

10.1016/0027-5107(81)90035-X

Klaassen CD, 1980, Casarett and Doull's Toxicology—The Basic Science of Poisons, 28

10.1016/0165-1110(81)90003-8

10.1093/carcin/10.6.1067

Maltoni C, 1986, Archives of Research on Industrial Carcinogenesis, 114

10.1016/0165-7992(83)90042-8

National Toxicology Program (1986):Toxicology and carcinogenesis studies of dichloromethane (methylene chloride; CAS No. 75‐09‐2) in F344 N rats and B6C3F1 mice (inhalation studies). Technical Report Series No. 306.

10.3109/10915818809019544

10.1016/0047-6374(81)90051-8

10.1093/mutage/2.1.57

10.1016/0165-1218(83)90074-5

10.1007/BF02623392

10.1002/em.2850100209

U.S. Environmental Protection Agency(1985):Health assessment document for dichloromethane (methylene chloride). Final Report EPA/600/8‐82/004F.

Westbrook‐CollinsB ShariefY CampbellJ AllenJW(1990):Further evidence that dichloromethane does not induce chromosome damage. J Appl Toxicol in press.

10.1007/BF00290991

Thông tin
Thông tin xuất bản

Environmental and Molecular Mutagenesis

Tập 15 Số 4

221-228

Thông tin tác giả