Cyclic diguanylate (c‐di‐GMP) regulates Vibrio cholerae biofilm formation

Molecular Microbiology - Tập 53 Số 3 - Trang 857-869 - 2004
Anna D. Tischler1, Andrew Camilli1
1Department of Molecular Biology and Microbiology Tufts University School of Medicine, 136 Harrison Avenue, Boston MA 02111, USA

Tóm tắt

SummaryWhile studying virulence gene regulation in Vibrio cholerae during infection of the host small intestine, we identified VieA as a two‐component response regulator that contributes to activating expression of cholera toxin. Here we report that VieA represses transcription of Vibrio exopolysaccharide synthesis (vps) genes involved in biofilm formation by a mechanism independent of its phosphorelay and DNA‐binding activities. VieA controls the intracellular concentration of the cyclic nucleotide second messenger cyclic diguanylate (c‐di‐GMP) using an EAL domain that functions as a c‐di‐GMP phosphodiesterase. Two‐dimensional thin layer chromatography of nucleotide extracts confirmed that VieA reduces the concentration of c‐di‐GMP, opposing the action of  c‐di‐GMP synthetase proteins. Expression of unrelated V. cholerae c‐di‐GMP synthetase or phosphodiesterae proteins also modulated c‐di‐GMP concentration and vps gene expression. We propose that c‐di‐GMP synthetase and phosphodiesterase domain‐containing proteins contribute to regulating biofilm formation by controlling c‐di‐GMP concentration.

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Molecular Microbiology

Tập 53 Số 3

857-869

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