Curcumin‐loaded poly(ε‐caprolactone) nanofibres: Diabetic wound dressing with anti‐oxidant and anti‐inflammatory properties

Clinical and Experimental Pharmacology and Physiology - Tập 36 Số 12 - Trang 1149-1156 - 2009
Jonathan G. Merrell1, S. McLaughlin2, Lu Tie3, Cato T. Laurencin4,5, Alex F. Chen6, Lakshmi S. Nair4,5
1Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA
2#N# †Department of Orthopaedic Surgery, University of Virginia, Charlottesville, Virginia
3Department of Pharmacology, Peking University Health Sciences Center, Beijing, China
4Department of Chemical, Materials and Biomolecular Engineering, University of Connecticut, Storrs, Connecticut
5Department of Orthopaedic Surgery, University of Connecticut Health Center, Farmington
6Pharmacology, Neurology, Cell and Molecular Biology, Neuroscience Program, Michigan State University, East Lansing, Michigan, USA

Tóm tắt

Summary Curcumin is a naturally occurring poly‐phenolic compound with a broad range of favourable biological functions, including anti‐cancer, anti‐oxidant and anti‐inflammatory activities. The low bioavailability and in vivo stability of curcumin require the development of suitable carrier vehicles to deliver the molecule in a sustained manner at therapeutic levels. In the present study, we investigated the feasibility and potential of poly(caprolactone) (PCL) nanofibres as a delivery vehicle for curcumin for wound healing applications. By optimizing the electrospinning parameters, bead‐free curcumin‐loaded PCL nanofibres were developed. The fibres showed sustained release of curcumin for 72 h and could be made to deliver a dose much lower than the reported cytotoxic concentration while remaining bioactive. Human foreskin fibroblast cells (HFF‐1) showed more than 70% viability on curcumin‐loaded nanofibres. The anti‐oxidant activity of curcumin‐loaded nanofibres was demonstrated using an oxygen radical absorbance capacity (ORAC) assay and by the ability of the fibres to maintain the viability of HFF‐1 cells under conditions of oxidative stress. The curcumin‐loaded nanofibres also reduced inflammatory induction, as evidenced by low levels of interleukin‐6 release from mouse monocyte–macrophages seeded onto the fibres following stimulation by Escherichia coli‐derived lipopolysaccharide. The in vivo wound healing capability of the curcumin loaded PCL nanofibres was demonstrated by an increased rate of wound closure in a streptozotocin‐induced diabetic mice model. These results demonstrate that the curcumin‐loaded PCL nanofibre matrix is bioactive and has potential as a wound dressing with anti‐oxidant and anti‐inflammatory properties.

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Thông tin xuất bản

Clinical and Experimental Pharmacology and Physiology

Tập 36 Số 12

1149-1156

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