Cross-regulation between Aurora B and Citron kinase controls midbody architecture in cytokinesis

Open Biology - Tập 6 Số 3 - Trang 160019 - 2016
Callum McKenzie1, Zuni I. Bassi1, Janusz Dębski2, Marco Gottardo3, Giuliano Callaini3, Michał Dadlez2, Pier Paolo D’Avino1
1Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
2Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Warszawa 02-106, Poland
3Department of Life Sciences, University of Siena, Via A. Moro 4, Siena 53100, Italy

Tóm tắt

Cytokinesis culminates in the final separation, or abscission, of the two daughter cells at the end of cell division. Abscission relies on an organelle, the midbody, which forms at the intercellular bridge and is composed of various proteins arranged in a precise stereotypic pattern. The molecular mechanisms controlling midbody organization and function, however, are obscure. Here we show that proper midbody architecture requires cross-regulation between two cell division kinases, Citron kinase (CIT-K) and Aurora B, the kinase component of the chromosomal passenger complex (CPC). CIT-K interacts directly with three CPC components and is required for proper midbody architecture and the orderly arrangement of midbody proteins, including the CPC. In addition, we show that CIT-K promotes Aurora B activity through phosphorylation of the INCENP CPC subunit at the TSS motif. In turn, Aurora B controls CIT-K localization and association with its central spindle partners through phosphorylation of CIT-K's coiled coil domain. Our results identify, for the first time, a cross-regulatory mechanism between two kinases during cytokinesis, which is crucial for establishing the stereotyped organization of midbody proteins.

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Open Biology

Tập 6 Số 3

160019

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