Costus speciosus leaf and seed extracts for wound healing: a comparative evaluation using mice excision wound models

Springer Science and Business Media LLC
Shafiul Azam Zishan1, Mir Md. Rokib Uddin1, Mohammad Arif M.2, Sanjay Azad1, Jannatul Naima3, Sadab Sipar Ibban4, Mitra Arman1
1Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong, 4318, Bangladesh
2Department of Chemistry, Chittagong University of Engineering and Technology, Chittagong, 4349, Bangladesh
3Department of Pharmacy, University of Chittagong, Chittagong 4331, Bangladesh
4Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Japan

Tóm tắt

Abstract Background The present study investigated the potential wound healing activity comparisons between ethanol leaf & seed extracts of Costus speciosus topical formulation using mice Excision wound models, compared to Neomycin sulphate ointment as a standard drug. To assess the efficacy of Costus speciosus ointment as a potential alternative to commercially available ointments, a study was conducted using a cohort of 16 healthy male mice, aged between 3 and 4 months. The ointment formulation was prepared utilizing ethanol extracts from both the leaves and seeds, presented in a dosage form for application. This investigation seeks to provide insights into the comparative effectiveness of Costus speciosus ointments in relation to conventional market preparations, with a focus on its potential applicability as an alternative therapeutic option. The parameter measured was wound contraction and epithelization period. Result The mice groups treated with seed & leave extracts ointment showed better wound size changes than the negative and standard groups. However, the leaf extract ointment promotes the formation of granulation in tissue, allowing the reepithelization phase to occur more rapidly than seed extract. From the 1st day to the results of the 9th day of the extract indicate that there is a significant increase (P < 0.05) in the percentage of wound contraction in the group. After 21 days the wound was healed fully. These results suggest that ointment of ethanol leaf extract of Costus speciosus could be an answer to facilitate wound healing compared to seed extract, to approve the traditional claims of the plant in wound healing activity. Conclusion The study concludes that ethanol leaf and seed extracts of Costus speciosus exhibit potential wound healing activity in mice excision wound models. The topical formulation of these extracts significantly enhances wound closure, reduces wound size, and increases tensile strength compared to the control group. The efficacy of the extracts is comparable to the standard drug, Neomycin sulphate ointment. Therefore, the use of Costus speciosus extracts in topical formulations can be considered a promising alternative for the treatment of wounds. However, further studies are needed to determine the safety and efficacy of these extracts in humans.

Từ khóa


Tài liệu tham khảo

Nandanwar R, Gurjar H, Sahu VK, Saraf H. Studies on wound healing activity of gel formulation containing cow ghee and Aloe vera. Int J Pharma Sci Res. 2010;1:50–4.

Shah SA, Sohail M, Khan S, Minhas MU, De Matas M, Sikstone V, Hussain Z, Abbasi M, Kousar M. Biopolymer-based biomaterials for accelerated diabetic wound healing: a critical review. Int J Biol Macromol. 2019;139:975–93.

Ambekar RS, Kandasubramanian B. Advancements in nanofibers for wound dressing: a review. Eur Polymer J. 2019;117:304–36.

El-Far A, Shaheen H, Alsenosy A, El-Sayed Y, Al Jaouni S, Mousa S. Costus speciosus: traditional uses, phytochemistry, and therapeutic potentials. Pharmacogn Rev. 2018;12(23).

Tyagi B, Gupta M. Ploidy status and diosgenin content in Costus speciosus. (Koen) Sm Cytologia. 1987;52(1):41–6.

de Albuquerque RDDG, Perini JA, Machado DE, Angeli-Gamba T, dos Santos Esteves R, Santos MG, et al. Wound Healing Activity Chem Stand Eugenia Pruniformis Cambess. 2016;12(48):288.

Sdayria J, Rjeibi I, Feriani A, Ncib S, Bouguerra W, Hfaiedh N et al. Chemical composition and antioxidant, analgesic, and anti-inflammatory effects of methanolic extract of Euphorbia retusa in mice. 2018;2018.

Criollo-Mendoza MS, Contreras-Angulo LA, Leyva-López N, Gutiérrez-Grijalva EP, Jiménez-Ortega LA, Heredia JB. Wound Healing Prop Nat Products: Mech Action. 2023;28(2):598.

Muluye AB, Desta AG, Abate SK, Dano GT. Anti-malarial activity of the root extract of Euphorbia abyssinica (Euphorbiaceae) against Plasmodium berghei infection in mice. Malar J. 2019;18(1):1–8.

Thakur R, Jain N, Pathak R, Sandhu SS. Practices in wound healing studies of plants. Evidence-based complementary and alternative medicine. 2011;2011.

Sharma A, Khanna S, Kaur G, Singh I. Medicinal plants and their components for wound healing applications. Future J Pharm Sci. 2021;7(1):1–13.

Majumder P, Taxo-cognostic. Phyto-Physicochemical & Biological screening of a plant: Taxo-cognostic, phytochemical, physicochemical parameters & Biological screening of Zyziphus Oenoplia (L.) Mill plant root. LAP LAMBERT Academic Publishing; 2012.

Gonzalez ACO, Costa TF, Andrade ZA, Medrado ARAP. Wound healing-A literature review. An Bras Dermatol. 2016;91:614–20.

Guo S, DiPietro LA. Factors affecting wound healing. J Dent Res. 2010;89(3):219–29.

Anderson K, Hamm RL. Factors that impair wound healing. J Am Coll Clin Wound Spec. 2012;4(4):84–91.

Banu N, Alam N, Nazmul Islam M, Islam S, Sakib SA, Hanif NB, et al. Insightful valorization of the biological activities of Pani Heloch leaves through experimental and computer-aided mechanisms. Molecules. 2020;25(21):5153.

Sawant SE, Tajane MD. Formulation and evaluation of herbal ointment containing neem and turmeric extract. J Sci Innovative Res. 2016;5(4):149–51.

Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain. 1983;16(2):109–10.

Charmeau-Genevois C, Sarang S, Perea M, Eadsforth C, Austin T, Thomas P. A simplified index to quantify the irritation/corrosion potential of chemicals–part I: skin. Regul Toxicol Pharmacol. 2021;123:104922.

Nayak B, Anderson M, Pereira LP. Evaluation of wound-healing potential of Catharanthus roseus leaf extract in rats. Fitoterapia. 2007;78(7–8):540–4.

Kokane DD, More RY, Kale MB, Nehete MN, Mehendale PC, Gadgoli CH. Evaluation of wound healing activity of root of Mimosa pudica. J Ethnopharmacol. 2009;124(2):311–5.

Kumar B, Vijayakumar M, Govindarajan R, Pushpangadan P. Ethnopharmacological approaches to wound healing—exploring medicinal plants of India. J Ethnopharmacol. 2007;114(2):103–13.

Mukherjee PK, Verpoorte R, Suresh B. Evaluation of in-vivo wound healing activity of Hypericum patulum (family: Hypericaceae) leaf extract on different wound model in rats. J Ethnopharmacol. 2000;70(3):315–21.

Alam N, Banu N, Aziz M, Ibn A, Barua N, Ruman U, et al. Chemical profiling, pharmacological insights and in Silico studies of methanol seed extract of Sterculia foetida. Plants. 2021;10(6):1135.

Paju N, Yamlean PV, Kojong N. Uji Efektivitas salep ekstrak daun binahong (Anredera cordifolia (ten.) Steenis) pada kelinci (Oryctolagus cuniculus) Yang Terinfeksi Bakteri Staphylococcus aureus. Pharmacon. 2013;2(1).

Leibovich S, Ross R. The role of the macrophage in wound repair. A study with hydrocortisone and antimacrophage serum. Am J Pathol. 1975;78(1):71.

Singer AJ, Clark RA. Cutaneous wound healing. N Engl J Med. 1999;341(10):738–46.

Phillips GD, Whitehead RA, Knighton DR. Initiation and pattern of angiogenesis in wound healing in the rat. Am J Anat. 1991;192(3):257–62.

Ahsan A, Miana G, Naureen H, RehmanA MU, Anum K, Malik I, Extraction. Phytochemical Screening and Wound Healing Activity of Herbal Formulation of Saussurea lappa: Wound Healing and Anti-bacterial Activity of Saussurea lappa. Proceedings of the Pakistan Academy of Sciences: B Life and Environmental Sciences. 2019;56(3):83-96-83–96.

Saraf A. Phytochemical and antimicrobial studies of medicinal plant Costus speciosus (Koen). J Chem. 2010;7:405–S13.

Coutinho MAS, Casanova LM, Nascimento LBS, Leal D, Palmero C, Toma HK et al. Wound healing cream formulated with Kalanchoe pinnata major flavonoid is as effective as the aqueous leaf extract cream in a rat model of excisional wound. 2021;35(24):6034–9.

Taddese SM, Gurji TB, Abdulwuhab M, Aragaw TJJE-BC, Medicine A. Wound healing activities of hydromethanolic crude extract and solvent fractions of Bersama abyssinica Leaves in mice. 2021;2021.

Lambebo MK, Kifle ZD, Gurji TB, Yesuf JSJJEP. Evaluation of wound healing activity of methanolic crude extract and solvent fractions of the leaves of Vernonia auriculifera Hiern (Asteraceae) in mice. 2021:677– 92.

Sasidharan S, Logeswaran S, Latha LYJI. Wound healing activity of Elaeis guineensis leaf extract ointment. 2011;13(1):336–47.

Alsareii SA, Alzerwi NA, Alasmari MY, Alamri AM, Mahnashi MH, Shaikh IA et al. Manilkara zapota L. extract topical ointment application to skin wounds in rats speeds up the healing process. 2023;14.

Islam R, Ullah A, Mukim M, Ashraf M, Haque R. Pharmacological investigation of the Wound Healing ActivitY of Costus Specious Plant2015.

Sohrab S, Mishra P, Mishra SKJBNRC. Phytochemical competence and pharmacological perspectives of an endangered boon—Costus speciosus. (Koen) Sm: Compr Rev. 2021;45(1):1–27.

Al-Attas AA, El-Shaer NS, Mohamed GA, Ibrahim SR. Esmat AJJoe. Anti-inflammatory sesquiterpenes from Costus speciosus rhizomes. 2015;176:365– 74.

Malabadi RBJJopr. Antibacterial activity in the rhizome extract of Costus speciosus. (Koen). 2005;18(1):83–5.

Thông tin
Thông tin xuất bản

Springer Science and Business Media LLC

Thông tin tác giả