Correlation between hormonal homeostasis and morphogenic responses in <i>Arabidopsis thaliana</i> seedlings growing in a Cd/Cu/Zn multi‐pollution context

Physiologia Plantarum - Tập 149 Số 4 - Trang 487-498 - 2013
Adriano Sofo1, Antonella Vitti1, Maria Nuzzaci1, Giuseppe Tataranni1, Antonio Scopa1, Jaco Vangronsveld2, Tony Remans2, Giuseppina Falasca3, Maria Maddalena Altamura3, Francesca Degola4, Luigi Sanità di Toppi4
1School of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, Viale dell’Ateneo Lucano 10, I-85100, Potenza, Italy
2Environmental Biology Centre for Environmental Sciences Hasselt University B‐3590 Diepenbeek Belgium
3Department of Environmental Biology “Sapienza” University of Rome, Piazzale A. Moro 5 I‐00185 Rome Italy
4Department of Life Sciences University of Parma, Parco Area delle Scienze I‐43124 Parma Italy

Tóm tắt

To date, almost no information is available in roots and shoots of the model plant Arabidopsis thaliana about the hierarchic relationship between metal accumulation, phytohormone levels, and glutathione/phytochelatin content, and how this relation affects root development. For this purpose, specific concentrations of cadmium, copper and zinc, alone or in triple combination, were supplied for 12 days to in vitro growing seedlings. The accumulation of these metals was measured in roots and shoots, and a significant competition in metal uptake was observed. Microscopic analyses revealed that root morphology was affected by metal exposure, and that the levels of trans‐zeatin riboside, dihydrozeatin riboside, indole‐3‐acetic acid and the auxin/cytokinin ratio varied accordingly. By contrast, under metal treatments, minor modifications in gibberellic acid and abscisic acid levels occurred. Real‐time polymerase chain reaction analysis of some genes involved in auxin and cytokinin synthesis (e.g. AtNIT in roots and AtIPT in shoots) showed on average a metal up‐regulated transcription. The production of thiol‐peptides was induced by all the metals, alone or in combination, and the expression of the genes involved in thiol‐peptide synthesis (AtGSH1, AtGSH2, AtPCS1 and AtPCS2) was not stimulated by the metals, suggesting a full post‐transcriptional control. Results show that the Cd/Cu/Zn‐induced changes in root morphology are caused by a hormonal unbalance, mainly governed by the auxin/cytokinin ratio.

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Thông tin xuất bản

Physiologia Plantarum

Tập 149 Số 4

487-498

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