Correlation between chlorophyllide esterification, Shibata shift and regeneration of protochlorophyllide650 in flash‐irradiated etiolated barley leaves

Physiologia Plantarum - Tập 121 Số 4 - Trang 556-567 - 2004
V. V. Rassadina1, В. П. Доманский1, Н. Г. Аверина1, Siegrid Schoch2, Wolfhart Rüdiger2
1Institute of Photobiology of the National Academy of Sciences of Belarus, Akademicheskaya 27, 220072 Minsk, Belarus
2Department of Biology I (Botanik), Ludwig-Maximilians-Universität München, Menzingerstr. 67, D-80638 München, Germany

Tóm tắt

The pigments of etiolated leaves of barley (Hordeum vulgare L.) were analysed during dark periods after flash illumination, and the results were compared with in vivo spectroscopy of the leaves. Pretreatment of the leaves with kinetin slightly stimulated and pretreatment with NaF and anaerobiosis inhibited the esterification of chlorophyllide a (Chlide) at 10–40 min after the flash, whereas the rapid esterification within 30 s after the flash remained unchanged. Irrespective of pretreatment, the amount of esterified pigment was, at any time, identical with the amount of pigment that had shifted its absorption from 684 to 672 nm (Shibata shift). Cycloheximide (CHI) had only a small inhibitory effect on esterification, but drastically inhibited the hydrogenation of geranylgeraniol to phytol, bound to Chlide. The regeneration of long‐wavelength protochlorophyllide a (Pchlide650) was stimulated by kinetin and inhibited by CHI and NaF. During the rapid phase (0–30 s after the flash), the esterification was faster than the regeneration of Pchlide650, and this, in turn, was faster than the formation of photoactive Pchlide. The kinetics changed after pretreatment with 5‐aminolaevulinic acid: regeneration of Pchlide650 was the fastest reaction and the Shibata shift preceded the esterification of Chlide. The results are discussed as pigment exchange reactions at NADPH:protochlorophyllide oxidoreductase (POR; EC 1.6.99.1).

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Physiologia Plantarum

Tập 121 Số 4

556-567

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