Conversion of Endogenous Indole-3-Butyric Acid to Indole-3-Acetic Acid Drives Cell Expansion in Arabidopsis Seedlings

Oxford University Press (OUP) - Tập 153 Số 4 - Trang 1577-1586 - 2010
Lucia C. Strader1,2, Angela Hendrickson Culler1,2, Jerry D. Cohen1,2, Bonnie Bartel1,2
1Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005 (L.C.S., B.B.)
2Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005 (L.C.S., B.B.); Department of Horticultural Science and Microbial and Plant Genomics Institute, University of Minnesota, St. Paul, Minnesota 55108 (A.H.C., J.D.C.)

Tóm tắt

AbstractGenetic evidence in Arabidopsis (Arabidopsis thaliana) suggests that the auxin precursor indole-3-butyric acid (IBA) is converted into active indole-3-acetic acid (IAA) by peroxisomal β-oxidation; however, direct evidence that Arabidopsis converts IBA to IAA is lacking, and the role of IBA-derived IAA is not well understood. In this work, we directly demonstrated that Arabidopsis seedlings convert IBA to IAA. Moreover, we found that several IBA-resistant, IAA-sensitive mutants were deficient in IBA-to-IAA conversion, including the indole-3-butyric acid response1 (ibr1) ibr3 ibr10 triple mutant, which is defective in three enzymes likely to be directly involved in peroxisomal IBA β-oxidation. In addition to IBA-to-IAA conversion defects, the ibr1 ibr3 ibr10 triple mutant displayed shorter root hairs and smaller cotyledons than wild type; these cell expansion defects are suggestive of low IAA levels in certain tissues. Consistent with this possibility, we could rescue the ibr1 ibr3 ibr10 short-root-hair phenotype with exogenous auxin. A triple mutant defective in hydrolysis of IAA-amino acid conjugates, a second class of IAA precursor, displayed reduced hypocotyl elongation but normal cotyledon size and only slightly reduced root hair lengths. Our data suggest that IBA β-oxidation and IAA-amino acid conjugate hydrolysis provide auxin for partially distinct developmental processes and that IBA-derived IAA plays a major role in driving root hair and cotyledon cell expansion during seedling development.

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Oxford University Press (OUP)

Tập 153 Số 4

1577-1586

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