Contribution of the microflora to proteolysis in the human large intestine
Tóm tắt
Protease activities in human ileal effluent were approximately 20‐fold greater than in normal faeces. Comparative studies with faeces from a person who did not have a pancreas suggested that a substantial proportion of the proteolytic activity in normal faeces was of bacterial origin. Thimerosal, iodoacetate, EDTA and cysteine significantly inhibited proteolysis in faeces, but not in small intestinal contents, showing that cysteine and metalloproteases were produced by bacteria in the large gut. These results, together with results from studies using
Từ khóa
Tài liệu tham khảo
Appel W., 1984, Methods of Enzymatic Analysis, 949
Cowan S.T., 1974, Cowan and Steel's Manual for the Identification of Medical Bacteria, 51
Cummings J. H., 1986, Proceedings of the XIV International Congress of Microbiology, 309
Gibson S.A.W., 1988, Studies on the proteolytic activity of Bacteroides fragilis, Journal of General Microbiology
Gibson S.A.W., 1986, Proceedings of the XIV International Congress of Microbiology, 274
Hentges D.J., 1985, Bacterial Enzymes and Virulence, 105
Holdeman L.V., 1977, Anaerobic Laboratory Manual, 30
Kaspar P., 1984, New photometric assay for chymotrypsin in stool, Clinical Chemistry, 30, 1753, 10.1093/clinchem/30.11.1753
Kopecny J., 1982, Cellular location and some properties of proteolytic enzymes of rumen bacteria, Applied and Environmental Microbiology, 43, 1026, 10.1128/aem.43.5.1026-1033.1982
Macfarlane G.T., 1986, Protein degradation by human intestinal bacteria, Journal of General Microbiology, 132, 1647
Nugent J.H.A., 1978, Rumen proteolysis of fraction 1 leaf protein, casein and bovine serum albumin, Proceedings of the Nutrition Society, 37, 48A
Wiggins H.S., 1983, Dietary Fibre, 205
Wrong O.M., 1981, The Large Intestine: its Role in Mammalian Nutrition and Homeostasis, 133