Construction and application a vane system in a rotational rheometer for determination of the rheological properties of Monascus ruber CCT 3802
Tóm tắt
The objectives of this work were the construction and adaptation of a vane system in a rotational rheometer; calibration of vane system using a mineral oil, and determination of rheological properties of the broth fermentation of Monascus ruber CCT 3802 at high cell density. Batch fermentation was carried out with glucose as the sole carbon source in a Bioflo III bioreactor. The consistency index (K) and flow behavior index (n) of the broth fermentation of M. ruber were characterized at different biomass concentrations and were adequately described by a power law model. The K and n values were significantly affected by biomass concentrations. K values ranged from 0.375 to 11.002 Pa s
n
when evaluated at biomass concentrations from 25.67 to 63.20 g L−1. The pseudoplastic behavior was confirmed by values of n that ranged between 0.157 and 0.254. Simple empirical correlations have been proposed to quantify the dependence of the power law terms on fungal biomass concentration.
Tài liệu tham khảo
Porcel EMR, López JLC, Pérez JAS, Sevilla JMF, Chisti Y. Effects of pellet morphology on broth rheology in fermentations of Aspergillus terreus. Biochem Eng J. 2005;26(2-3):139–44.
Schmidell W. Transferência de oxigênio em biorreatores. In: Schmidell W, Borzani W, Lima UA, Aquarone E, editors. Biotecnologia industrial. São Paulo: Edgard Blücher; 2001. p. 341–61.
Goudar CT, Strevett KA, Shah SN. Influence of microbial concentration on the rheology of non-Newtonian fermentation broths. Appl Microbiol Technol. 1999;51(3):310–5.
Sinha JJT, Bae JP, Park CH, Song CH, Yun JW. Effect of substrate concentration on broth rheology and fungal morphology during exo-biopolymer production by Paecilomyces japonica in a batch bioreactor. Enzyme Microbiol Technol. 2001;29(6):392–9.
Riely GL, Tucker KG, Thomas CR. Effect of biomass concentration and mycelial morphology on fermentation broth rheology. Biotechnol Bioeng. 2000;68(2):160–72.
Sánchez MJ, Aparício AJ, López GG, Tapia GT, Monroy MR. Broth rheology of Beta vulgaris cultures growing in an air lift bioreactor. Biochem Eng J. 2002;12(1):37–41.
Lim SJ, Kim JH, Kim C, Kim SW. Morphological and rheological properties of culture broth of Cephalosporium acremonium M25. Korean Aust Rheol J. 2002;14(1):11–6.
Suijdam JC, Metz B. Influence of engineering variables upon the morphology of filamentous moulds. Biotechnol Bioeng. 1981;23(1):111–48.
Berovic M, Koloini T, Olsvik ES, Kristiansen B. Rheological and morphological properties of submerged citric acid fermentation broth in stirred-tank and bubble column reactors. Chem Eng J. 1993;53(2):35–40.
Cho YJ, Hwang HJ, Kim SW, Song CH, Yun JW. Effect of carbon source and aeration rate on broth rheology and fungal morphology during red pigment production by Paecilomyces sinclairii in a batch bioreactor. J Biotechnol. 2002;95(1):13–23.
Reuss M, Debus D, Zoll G. Rheological properties of fermentation fluids. Chem Eng J. 1982;183:233–6.
Allen DG, Robinson CW. Measurements of rheological properties of filamentous fermentation broths. Chem Eng Sci. 1990;45:37–48.
Li GQ, Qiu HW, Zheng ZM, Cai ZL, Yang SZ. Effect of fluid rheological properties on mass transfer in a bioreactor. Chem Technol Biotechnol J. 1995;62:385–91.
Badino ACJr, Facciotti MCR, Schmidell W. Estimation of the rheology of glucoamylase fermentation broth from the biomass concentration and shear conditions. Biotechnol Tech. 1999;13:723–6.
Rao MA. Rheology of fluid and semisolid foods: principles and applications. Gaithersburg: Aspen; 1999.
Badino ACJr, Facciotti MCR, Schmidell W. Construction and operation of an impeller rheometer on-line rheological characterization of non-Newtonian fermentation broths. Braz J Chem Eng. 1997;14(4):359–65.
Metzner AB, Otto RE. Agitation of non-Newtonian fluids. Am Int Chem J. 1957;3(1):3–10.
Bongenaar JJTM, Kossen NWF, Tart KR. A method for characterization of the rheological properties of viscous fermentation broths. Biotechnol Bioeng. 1973;15:201–6.
Kemblowski Z, Kristiansen B. Rheometry of fermentation liquids. Biotechnol Bioeng. 1986;28:1474–83.
Rushton JH, Costich EW, Everett HJ. Power characteristics of mixing impellers—part 1. J Chem Eng Prog. 1950;46(8):395–404.
Calderbank PH, Moo-Youn MB. The prediction of power consumption in the agitation of non-Newtonian fluids. Trans Inst Chem Eng. 1959;37:26–33.
Queiroz MCR, Facciotti MCR, Schmidell W. Rheological changes of Aspergillus awamori broth during amyloglucosidase. Biotechnol Lett. 1997;19(2):167–70.
Kayacier A, Dogan M. Rheological properties of some gums-salep mixed solutions. J Food Eng. 2006;72:261–5.
Roels JA, van den Berg J, Voncken RM. The rheology of mycelial broths. Biotechnol Bioeng. 2004;16(2):181–208.
Pollard DJ, Hunt G, Kirschner TK, Salmon PM. Rheological characterization of a fungal fermentation for the production of pneumocadins. Bioprocess Biosyst Eng. 2002;24:373–83.
Olsvik ES, Kristiansen B. Rheology of filamentous fermentations. Biotechnol Adv. 1994;12:1–39.
Ballica R, Ruy D. Effects of rheological properties and mass transfer on plant cell bioreactor performance: production of tropane alkaloids. Biotechnol Bioeng. 1993;42:1181–9.
Zhang Y, Wang H, Liu S, Yu J, Zhong J. Regulation of apparent viscosity and O2 transfer coefficient by osmotic pressure in cell suspension of Panax notoginseng. Biotechnol Lett. 1997;19(10):943–5.