Constant and intermittent high glucose enhances endothelial cell apoptosis through mitochondrial superoxide overproduction
Tóm tắt
It has been previously shown that hyperglycemia enhances free radical production, inducing oxidative damage, which in its turn activates the death pathways implicated in cell apoptosis and necrosis. But the possible involvement of this pathway in the hyperglycemia‐induced apoptosis of endothelial cells has not yet been reported.
To verify a possible connection between mitochondrial ROS production and apoptosis induced by both stable and oscillating high glucose, SOD, MnTBAP and TTFA was added to HUVEC cell culture medium. We measured nitrotyrosine and 8OHdG as oxidative stress parameters and Bcl‐2 expression and Caspase‐3 expression and activity as apoptosis indicators.
Our results show that hyperglycemia, both stable or oscillating, increases oxidative stress and endothelial cell apoptosis through ROS overproduction at the mitochondrial transport chain level.
The prevention of mitochondrial oxidative damage seems to be a future important therapeutic strategy in diabetes. Copyright © 2006 John Wiley & Sons, Ltd.
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Tài liệu tham khảo
Takeuchi A, 1995, Periodic high extracellular glucose enhances production of collagens III and IV by mesangial cells, Am J Physiol, 268, F13
Kesavulu MM, 2000, Lipid peroxidation and antioxidant enzyme levels in type 2 diabetics with microvascular complications, Diabetes Metab, 26, 387
Ihnat MA, 2005, Reduced antioxidant Response of retinal and endothelial cells in response to cronic oscillating glucose levels, Diabetes, 52, A557