Conformation change of bovine serum albumin induced by bioactive titanium metals and its effects on cell behaviors

Journal of Biomedical Materials Research - Part A - Tập 102 Số 4 - Trang 1053-1062 - 2014
Xiaoyu Hu1,2, Bang Cheng Yang1,2
1Engineering Research Center in Biomaterials, Sichuan University, Chengdu 610064, China
2National Engineering Research Center for Biomaterials, Chengdu 610064, China

Tóm tắt

The conformation change of bovine serum albumin (BSA) induced by bioactive titanium surfaces, including acid‐alkali‐treated titanium (AA‐Ti) and alkali–heat‐treated titanium (AH‐Ti), was studied, and its effects on the activity of MC3T3‐E1 cell were evaluated. Pure titanium metal (P‐Ti) was used as control. The AA‐Ti could adsorb more BSA on its surface than AH‐Ti and P‐Ti. The α‐helix part of the protein adsorbed on P‐Ti has weakly decreased compared with native BSA, and it dramatically decreased on AA‐Ti and AH‐Ti. The β‐sheet segment of proteins adsorbed on P‐Ti and AH‐Ti had obviously increased. Much more tryptophan residues were exposed after the protein conformation changed when it interacted with AH‐Ti, and some tryptophan residues were enveloped after it interacted with AA‐Ti and P‐Ti. AA‐Ti has more tryptophan residues enveloped than P‐Ti. All titanium surfaces induced tyrosine residues exposed, especially for the P‐Ti. The higher ratio of COO−/NH3+ for the proteins on P‐Ti and AA‐Ti indicated an orientation of proteins on P‐Ti and AA‐Ti, which makes more COO− exposed. The lower ratio of COO−/NH3+ on AH‐Ti indicates that more NH3+ is exposed on its surface. The cell proliferation ability on different treated titanium surfaces coated with BSA followed by the order: P‐Ti > AA‐Ti > AH‐Ti, which indicated that the protein conformation change on different bioactive titanium surfaces has great effect on the cell activity. Our results showed that the different biological response of bioactive titanium metals might depend on the protein conformation change induced by the surface structure. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1053–1062, 2014.

Từ khóa


Tài liệu tham khảo

10.1111/j.1551-2916.2004.01635.x

10.1016/j.jeurceramsoc.2006.04.015

Rack MLaHJ, 1998, Titanium alloys in total joint replacement—A materials science perspective, Biomaterials, 19, 19

10.1016/j.mser.2004.11.001

10.1016/S0142-9612(03)00626-4

10.1023/A:1018524731409

10.1016/S0142-9612(97)82297-1

10.1016/j.biomaterials.2005.12.014

10.1016/S0142-9612(02)00548-3

10.1289/ehp.94102s541

Ballet T, 2010, Protein conformational changes induced by adsorption onto material surfaces: An important issue for biomedical applications of material science, Bull Pol Acad Sci: Tech Sci, 58, 303

10.1098/rsta.2011.0266

10.1016/S0142-9612(98)00184-7

10.1002/jbm.a.32561

10.1021/ja056278e

10.1006/jcis.1996.4750

10.1002/bip.10558

10.1016/j.jpba.2005.11.037

10.1177/154405910408300606

10.1111/j.1151-2916.1996.tb08561.x

10.1016/j.biomaterials.2003.08.009

10.1016/S0142-9612(01)00159-4

10.1021/ja01269a023

10.1016/j.actbio.2010.01.007

10.1002/(SICI)1097-4636(199911)47:2<213::AID-JBM11>3.0.CO;2-C

10.1016/j.foodhyd.2004.10.012

10.1016/S0142-9612(97)00223-8

10.1006/jcis.1999.6576

10.1016/j.bmc.2008.01.060

10.1021/bi00489a038

10.1016/S0022-5193(05)80132-8

10.1016/j.ijpharm.2007.09.016

10.1016/j.saa.2011.07.073

10.1073/pnas.150236197

10.1016/j.colsurfa.2008.04.017

10.1007/s13758-012-0048-4

10.1002/jbm.a.30096

10.1016/S0168-1656(00)00242-X

10.1016/j.actbio.2012.12.002

10.1007/BF02932892

10.1098/rsif.2010.0129.focus

10.1007/s10856-011-4372-x

10.1111/j.1751-1097.1973.tb06422.x

Thông tin
Thông tin xuất bản

Journal of Biomedical Materials Research - Part A

Tập 102 Số 4

1053-1062

Thông tin tác giả