Conditions for detecting the mutagenicity of divalent metals in Salmonella typhimurium

Environmental and Molecular Mutagenesis - Tập 19 Số 2 - Trang 139-146 - 1992
Dennis A. Pagano1, Errol Zeiger1
1Experimental Toxicology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina

Tóm tắt

Abstract

The mutagenesis of metals in bacteria, as reported in the literature, can best be described as inconsistent. We report that cobalt chloride (Co++), ferrous sulfate (Fe++), manganese sulfate (Mn++), cadmium chloride (Cd++), and zinc chloride (Zn++) could be reproducibly detected as mutagens in Salmonella strain TA97 when preincubation exposures were made in sterile, distilled, deionized water, or in Hepes buffer in NaCl2/KCl2, rather than the standard sodium phosphate buffer. Co++ was also mutagenic under standard preincubation conditions. The individual components of Vogel‐Bonner medium, i.e., potassium and ammonium phosphates, citrate, and magnesium sulfate, inhibit mutagenesis by these metals. The phosphates and the citrate probably inhibit by chelating the metals, while data are presented to suggest that Mg++ inhibition of metal mutagenesis is due to competitive inhibition for active transport via the magnesium active transport system in Salmonella. The chelator, diethyldithiocarbamate, inhibited the mutagenicity of Co++, Fe++, Zn++, and Mn++, but enhanced the mutagenicity of Cd++. The results presented show that divalent metals can be detected as mutagens in Salmonella, and that their lack of detection as mutagens is not due to an inherent insensitivity of Salmonella but to their interaction with media components and/or passive and active transport processes.

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Tài liệu tham khảo

Abelson PH, 1950, Ion antagonisms in microorganisms: interference of normal magnesium metabolism by nickel, cobalt, cadmium, zinc, and manganese, J Bacteriol, 60, 401, 10.1128/jb.60.4.401-413.1950

10.1016/0013-9351(85)90032-5

10.1128/MMBR.51.4.509-518.1987

10.1016/0165-1161(76)90110-2

10.1016/0165-1110(84)90009-5

10.1101/SQB.1951.016.01.017

10.1073/pnas.83.21.8268

10.1007/BF01868686

Hassan HM, 1977, Enzymatic defense against the toxicity of oxygen and of streptonigrin in Escherichia coli, J Bacteriol, 129, 1574, 10.1128/jb.129.3.1574-1583.1977

10.1002/em.2860050703

10.1016/0165-1218(79)90164-2

10.1128/jb.168.3.1444-1450.1986

10.1016/0006-291X(88)90480-9

10.1016/0300-483X(80)90107-9

10.1016/0165-1218(80)90127-5

Laddaga RA, 1985, Cadmium uptake in Escherichia coli K‐12, J Bacteriol, 162, 1100, 10.1128/jb.162.3.1100-1105.1985

10.1016/0027-5107(82)90294-9

10.1016/0165-1218(84)90079-X

10.1016/0165-1161(83)90010-9

Martin RB, 1983, Metal Ions in Biological Systems, 21

10.1016/0165-1218(85)90024-2

10.1016/0165-1218(83)90059-9

10.1002/em.2860080702

10.1073/pnas.69.5.1091

10.1016/0165-1161(75)90088-6

10.1016/0165-1218(88)90081-X

10.1016/0165-1218(86)90068-6

10.1128/JB.104.2.635-639.1970

10.1002/em.2860060108

10.1080/02772248709357209

Silver S, 1970, Manganese active transport in Escherichia coli, J Bacteriol, 104, 1299, 10.1128/jb.104.3.1299-1306.1970

Snavely MD, 1991, Magnesium transport in Salmonella typhimurium, J Biol Chem, 266, 824, 10.1016/S0021-9258(17)35247-X

Thompson ED, 1989, Genotoxicity of zinc in 4 short‐term mutagenicity assays, Radiat Res, 223, 267

10.1016/S0021-9258(18)65874-0

Westman G, 1980, Diethyldithiocarbamate, a superoxide dismutase inhibitor, decreases the radioresistance of Chinese hamster cells, Mutat Res, 83, 303

10.1016/0027-5107(84)90164-7

10.1002/em.2850110602

Zeiger E, 1992, Salmonella mutagenicity tests: V. Results from the testing of 311 chemicals, Environ Mol Mutagen, 19

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Environmental and Molecular Mutagenesis

Tập 19 Số 2

139-146

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