Comprehensive MicroRNA Profiling Reveals a Unique Human Embryonic Stem Cell Signature Dominated by a Single Seed Sequence

Stem Cells - Tập 26 Số 6 - Trang 1506-1516 - 2008
Louise C. Laurent1,2, Jing Chen3, Igor Ulitsky4, Franz‐Josef Müller2,5, Christina Lu1,2, Ron Shamir4, Jian‐Bing Fan3, Jeanne F. Loring2
1Department of Reproductive Medicine, University of California San Diego, San Diego, California, USA
2The Scripps Research Institute, La Jolla, California, USA
3Illumina, Inc., San Diego, California, USA
4School of Computer Science, Tel Aviv University, Tel Aviv, Israel
5Zentrum für Integrative Psychiatrie, Universitätsklinikums Schleswig-Holstein, Kiel, Germany

Tóm tắt

Abstract Embryonic stem cells are unique among cultured cells in their ability to self-renew and differentiate into a wide diversity of cell types, suggesting that a specific molecular control network underlies these features. Human embryonic stem cells (hESCs) are known to have distinct mRNA expression, global DNA methylation, and chromatin profiles, but the involvement of high-level regulators, such as microRNAs (miRNA), in the hESC-specific molecular network is poorly understood. We report that global miRNA expression profiling of hESCs and a variety of stem cell and differentiated cell types using a novel microarray platform revealed a unique set of miRNAs differentially regulated in hESCs, including numerous miRNAs not previously linked to hESCs. These hESC-associated miRNAs were more likely to be located in large genomic clusters, and less likely to be located in introns of coding genes. hESCs had higher expression of oncogenic miRNAs and lower expression of tumor suppressor miRNAs than the other cell types. Many miRNAs upregulated in hESCs share a common consensus seed sequence, suggesting that there is cooperative regulation of a critical set of target miRNAs. We propose that miRNAs are coordinately controlled in hESCs, and are key regulators of pluripotence and differentiation. Disclosure of potential conflicts of interest is found at the end of this article.

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