Comprehensive Mapping of Long-Range Interactions Reveals Folding Principles of the Human Genome

American Association for the Advancement of Science (AAAS) - Tập 326 Số 5950 - Trang 289-293 - 2009
Erez Lieberman-Aiden1,2,3,4, Nynke L. van Berkum5, Louise Williams1, Maxim Imakaev3, Tobias Ragoczy6,7, Agnes Telling6,7, Ido Amit1, Bryan R. Lajoie5, Peter J. Sabo8, Michael O. Dorschner8, Richard Sandstrom8, B Bernstein1,9, M. A. Bender10, Mark Groudine6,7, Andreas Gnirke1, J Stamatoyannopoulos8, Leonid A. Mirny11,3, Eric S. Lander1,12,13, Job Dekker5
1Broad Institute of Harvard and Massachusetts Institute of Technology (MIT), MA 02139, USA.
2Department of Applied Mathematics, Harvard University, Cambridge, MA 02138, USA.
3Division of Health Sciences and Technology, MIT, Cambridge, MA 02139, USA
4Program for Evolutionary Dynamics, Department of Organismic and Evolutionary Biology, Department of Mathematics, Harvard University, Cambridge, MA 02138, USA
5Program in Gene Function and Expression and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
6Department of Radiation Oncology, University of Washington School of Medicine, Seattle, WA 98195 USA
7Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
8Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
9Department of Pathology, Harvard Medical School, Boston, MA 02115 USA
10Department of Pediatrics, University of Washington, Seattle, WA 98195 (USA)
11Department of Physics, MIT, Cambridge, MA 02139, USA
12Department of Biology, MIT, Cambridge, MA 02139, USA
13Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA

Tóm tắt

Chromosomal Mapping

The conformation of the genome in the nucleus and contacts between both proximal and distal loci influence gene expression. In order to map genomic contacts, Lieberman-Aiden et al. (p. 289 , see the cover) developed a technique to allow the detection of all interactions between genomic loci in the eukaryotic nucleus followed by deep sequencing. This technology was used to map the organization of the human genome and to examine the spatial proximity of chromosomal loci at one megabase resolution. The map suggests that the genome is partitioned into two spatial compartments that are related to local chromatin state and whose remodeling correlates with changes in the chromatin state.

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