Complete sequence of a cDNA encoding an active rat choline acetyltransferase: A tool to investigate the plasticity of cholinergic phenotype expression

Journal of Neuroscience Research - Tập 23 Số 3 - Trang 266-273 - 1989
Alexis Brice1, Sylvie Berrard1, Brigitte Raynaud2, Stéphane Ansieau1, Thierry Coppola1, Michel Weber2, Jacques Mallet1
1Laboratoire de Neurobiologie Cellulaire et Moléculaire, Centre National de la Recherche Scientifique, Gif sur Yvette, France
2Laboratoire de Pharmacologie et de Toxicologie Fondamentales, Centre National de la Recherche Scientifique, Toulouse, France.

Tóm tắt

Abstract

A cDNA clone encoding the complete sequence of an active, rat choline acetyltransferase (ChoAcTase; acetyl‐CoA:choline O‐acetyltransferase, EC 2.3.1.6) has been isolated. Analysis of the deduced amino acid sequence reveals 85% and 31% identity with the porcine and Drosophila melanogaster enzymes, respectively. To further elucidate the molecular basis of neurotransmitter‐related phenotypic plasticity, the expression of ChoAcTase mRNA was compared with that of tyrosine hydroxylase [TH; tyrosine 3‐monooxygenase, L‐tyrosine, tetrahydropteridine: oxygen oxidoreductase (3‐hydroxylating), EC 1.14.16.2], in neurons from superior cervical ganglia grown in the following conditions: (1) normal medium, (2) high K+ medium, and (3) normal medium supplemented with 50% muscle‐conditioned medium (CM). TH mRNA was expressed in all three media; its level rose in high K+ and decreased strikingly in the presence of CM. ChoAcTase mRNA could be visualized in CM, but fell to undetectable levels in normal and high K+ media. These results suggest that translational or post‐translational mechanisms do not play a major role for the modulation of neurotransmitter‐associated phenotype.

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Journal of Neuroscience Research

Tập 23 Số 3

266-273

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