Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440

Wiley - Tập 4 Số 12 - Trang 799-808 - 2002
William Nelson1, Christian Weinel2, Ian T. Paulsen1, Robert J. Dodson1, H. Hilbert3, Vítor A. P. Martins dos Santos4, Derrick E. Fouts1, Steven R. Gill1, Mihai Pop1, Megan E. Holmes1, Lauren Brinkac1, Maureen J. Beanan1, Robert T. DeBoy1, Sean C. Daugherty1, James F. Kolonay1, Ramana Madupu1, Owen White1, Jeremy Peterson1, Hoda Khouri1, Ioana R. Hance1, P. Chris Lee1, Erik Holtzapple1, David J. Scanlan1, Kevin Tran1, A. Moazzez1, Teresa R. Utterback1, Michael Rizzo1, K. Lee1, D. Kosack1, D. Moestl3, Holger Wedler3, Jürgen Lauber3, D. Stjepandic5, Jörg D. Hoheisel6, M. Straetz4, Sabina Heim4, Claudia Kiewitz2, Jonathan A. Eisen1, Kenneth N. Timmis4, Andreas Düsterhöft3, Burkhard Tümmler2, Claire M. Fraser1
1J. Craig Venter Institute
2Klinische Forschergruppe
3Qiagen
4Helmholtz Centre for Infection Research (HZI)
5German Cancer Research Center (dkfz
6Deutsches Krebsforschungszentrum (DKFZ), Heidelberg, Germany

Tóm tắt

Summary

Pseudomonas putida is a metabolically versatile saprophytic soil bacterium that has been certified as a biosafety host for the cloning of foreign genes. The bacterium also has considerable potential for biotechnological applications. Sequence analysis of the 6.18 Mb genome of strain KT2440 reveals diverse transport and metabolic systems. Although there is a high level of genome conservation with the pathogenic Pseudomonad Pseudomonas aeruginosa (85% of the predicted coding regions are shared), key virulence factors including exotoxin A and type III secretion systems are absent. Analysis of the genome gives insight into the non‐pathogenic nature of P. putida and points to potential new applications in agriculture, biocatalysis, bioremediation and bioplastic production.

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Wiley

Tập 4 Số 12

799-808

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