Compartmentation and fluxes of inorganic phosphate in photosynthetic cells

Planta - Tập 161 - Trang 525-530 - 1984
Ian E. Woodrow1, J. Raymond Ellis1, Anita Jellings2, Christine H. Foyer1
1ARC Research Group on Photosynthesis, Department of Botany, University of Sheffield, Sheffield, UK
2Department of Biology, University of York, York, UK

Tóm tắt

An analysis of the compartmentation and fluxes of inorganic phosphate in isolated cladophyll cells from Asparagus officinalis was made in parallel with an ultrastructural study. The elution pattern of labelled inorganic phosphate (which indicates that the asparagus cells are behaving as a system of three compartments in series) was used to quantify the fluxes between the vacuole, cytoplasm and free space. A relaxation time of 198 min was calculated for inorganic phosphate exchange between the vacuole and cytoplasm. It is, therefore, suggested that the vacuole serves to buffer the cytoplasmic inorganic phosphate concentration in the long term. However, in the short term, exchange with the vacuole will not appreciably affect the cytoplasmic inorganic phosphate concentration and thus the partitioning of photosynthetically fixed carbon.

Tài liệu tham khảo

Bieleski, R.L. (1968) Levels of phosphate esters in Spirodela. Plant Physiol. 43, 1297–1308 Bieleski, R.L. (1969) Phosphorous compounds in translocating pholem. Plant Physiol. 44, 497–502 Bieleski, R.L. (1973) Phosphate pools, phosphate transport and phosphate availability. Annu. Rev. Plant Physiol. 24, 225–253 Bieleski, R.L., Laties, G.G. (1963) Turnover rates of phosphate esters in fresh and aged slices of potato tuber tissue. Plant Physiol. 38, 586–594 Colman, B., Mawson, B.T., Espie, G.S. (1979) The rapid isolation of photosynthetically active mesophyll cells from Asparagus cladophylls. Can. J. Bot. 57, 1505–1510 Cram, W.J. (1968) Compartmentation and exchange of chloride in carrot root tissue. Biochim. Biophys. Acta 163, 339–353 Cram, W.J. (1975) Storage tissues. In: Ion transport in plant cells and tissues. Barker, D.A., Hall, J.L., eds. North Holland Publishing Co., Amsterdam London Cséke, C., Buchanan, B.B. (1983) An enzyme synthesising fructose-2,6-bisphosphate occurs in leaves and is regulated by metabolic effectors. FEBS Lett. 155, 139–142 Findlay, G.P., Hope, A.B., Walker, N.A. (1971) Quantisation of a flux ratio in chlorophytes. Biochim. Biophys. Acta 233 155–162 Foyer, C.H., Walker, D.A., Spencer, C., Mann, B. (1982) Observations on the phosphate status and intracellular pH of intact cells protoplasts and chloroplasts from photosynthetic tissue using phosphorus-31 nuclear magnetic resonance. Biochem. J. 202, 429–434 Greenway, H., Klepper, B. (1968) Phosphorous transport to the xylem and its regulation by water flow. Planta 83, 119–136 Harbron, S., Foyer, C.H., Walker, D.A. (1981) The purification and properties of sucrose-phosphate synthetase from spinach leaves: the involvement of this enzyme and fructose bisphosphatase in the regulation of sucrose biosynthesis. Arch. Biochem. Biophys. 212, 237–246 Jullien, M. (1983) Medium-term preservation of mesophyll cells isolated from Asparagus officinalis L.: development of a simple method by storage at reduced temperature. Plant Cell Tissue Organ Culture 2, 305–316 Leech, R.M. (1977) Subcellular fractionation techniques in enzyme distribution studies. In: Regulation of enzyme synthesis and activity in higher plants, pp. 289–327, Smith, H., ed. Academic Press, London Mackinney, G. (1941) Absorption of light by chlorophyll solutions. J. Biol. Chem. 140, 315–323 MacRobbie, E.A.C. (1969) Ion fluxes to the vacuole of Nitella translucens. J. Exp. Bot. 20, 236–256 Pallaghy, C.K., Scott, B.I.H. (1969) The electrochemical state of cells of broad bean roots. II. Potassium kinetics in excised root tissue. Aust. J. Biol. Sci. 22, 585–600 Pitman, M.G. (1963) The determination of the salt relations of the cytoplasmic phase in cells of beetroot tissue. Aust. J. Biol. Sci. 16, 647–668 Rebeille, F., Bligny, R., Martin, J.-B., Douce, R. (1983) Relationship between the cytoplasm and the vacuole phosphate pool in Acer pseudoplatanus cells. Arch. Biochem. Biophys. 225, 143–148 Reynolds, E.S. (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 Spurr, A.R. (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–43 Tolbert, N.E., Weibe, H. (1955) Phosphorous and sulphur compounds in plant xylem sap. Plant Physiol 30, 499–504 Walker, D.A. (1980) Physiological aspects of crop productivity. Proc. 15th Colloq. Int. Potash Institute, Bern, pp. 195–207 Walker, N.A., Pitman, M.G. (1976) Measurement of fluxes across membranes. In: Encyclopedia of plant physiology, N.S., vol. 2A: Transport in plants. IIA. Cells, pp. 93–126, Lüttge, U., Pitman, M.G., eds. Springer, Berlin Heidelberg New York Weibel, E.R., Kistler, G.S., Scherle, W.F. (1966) Practical stereological methods for morphometric cytology. J. Cell Biol. 30, 23–38 Woodrow, I.E., Murphy, D.J., Walker, D.A. (1983) Regulation of photosynthetic carbon metabolism. The effect of inorganic phosphate on stromal sedoheptulose 1,7-bisphosphatase. Eur. J. Biochem. 132, 121–123