Comparison of two diagnostic tests for canine atopy using monoclonal anti‐IgE antibodies
Tóm tắt
In 66 dogs of various races consulting the veterinary clinic for skin problems suspicious of atopic dermatitis, parallel skin tests with two commercial brands of allergens and serological tests with the CMG IMMUNODOT system and with an ELISA assay using monoclonal anti‐IgE antibodies were performed. The most frequent sensitivities found were towards house dust (44%) and storage mites (50%). Depending upon the cut‐off point chosen, the CMG IMMUNODOT test was shown to have sensitivity, in respect to the skin test, varying from 54 to 100%, in an inverse relationship to specificity. The ELISA assay was found to be slightly less sensitive. These investigations also revealed some major discrepancies in skin test results among allergen extracts of different origins, confirming that skin test results should no longer be considered as the sole ‘gold standard’ in diagnosis of IgE‐mediated allergy. The experience gained confirms that modern serological tests using monoclonal anti‐IgE antibodies for detection of allergen‐specific IgEs in the dog are useful in the diagnosis of dog allergy.
Từ khóa
Tài liệu tham khảo
Scott D.W. Miller W.H. Griffin C.E.Miller & Kirk’s Small Animal Dermatology 5th edn. London: Saunders 1995.
Haliwell R.E.W., 1972, Antigenic relations between human IgE and canine IgE., Clinical Experimental Immunology, 10, 339
Hill P.B., 1994, Quantification of serum total IgE concentration in dogs by use of an enzyme‐linked immunosorbent assay containing monoclonal murine anti‐canine IgE., American Journal of Veterinary Research, 55, 944, 10.2460/ajvr.1994.55.07.944
Griot‐Wenk M.E. Marti E. Racine B. Crameri R. Zurbriggen A. De Weck A.L. Lazary S.Character‐ization of canine IgE‐specific chicken antibodies elicited by two recombinant fragments of the epsilon chain.American Journal of Veterinary Research1998; in press.
Kleinbeck M.L., 1989, Enzyme‐linked immunosorbent assay for measurement of allergen‐specific IgE anti‐bodies in canine serum., American Journal of Veterinary Research, 50, 1831
Peng Z., 1993, Measurement of ragweed‐specific IgE in canine serum by use of enzyme‐linked immunosorbent assays, containing polyclonal and monoclonal antibodies., Journal of Veterinary Research, 54, 239
Bigler B. De Weck A.L. Stadler B.M. Frick O.L. De Boer D.J. Derer M.Use of monoclonal anti‐IgE antibodies for the development of diagnostic tests in dog atopy.(Abstract) Proceedings of the 10th ESVD Congress Aalborg 1993:734.
de Weck A.L.Le diagnostic in vitro des allergies du chien.In: Bernard M. ed.Pathologie Vétérinaire et Allergie.Journées parisiennes d’aallergie. France: Bayer 1994:3146.
Codner E.C., 1993, Comparison of intradermal allergy test and enzyme‐linked immunosorbent assay in dogs with allergic skin disease., Journal of the American Veterinary Medicine Association, 202, 739, 10.2460/javma.1993.202.05.739
Miller W.H., 1992, Evaluation of an allergy screening test for use of atopic dogs., Journal of the American Veterinary Medicine Association, 200, 931, 10.2460/javma.1992.200.07.931
de Weck A.L., 1995, Top Screen. Ein neues Verfahren für die primäre Allergieabklärung., Allergologie, 18, 234
Bigler B., 1996, Messung von allergenspezifischen IgE beim Hund mit einem in vitro Test mit monoclonalem anti‐IgE Antikörper. Vergleich mit dem Intrakutantest und erste Ergebnisse der Hyposensibilisierung., Kleintierpraxis, 41, 643
Hasegawa A. Tsujimoto H. Ogata M. Iwasaki T. Sako T. Kuyama T. Yonekura T. Hashiguchi M. Sakaguchi M.Clinical evaluation of Top Screen and Immunodot assays. Diagnosis of canine allergic dermatitis by measurement of allergen‐specific IgE antibodies in dog serum.Japanese Journal of Veterinary Medicine1998: in press.
Perkins J., 1997, Diagnosis of atopy in dogs: a review of the need for conceptual and diagnostic refinement., Australian Veterinary Practitioner, 27, 8
De Boer D.J., 1989, Survey of intradermal skin testing practices in North America., Journal of the American Veterinary Medicine Association, 195, 1357
De Weck A.L. Derer M. Mayer P. Stumper B.Allergy starts in early life: clinical and experimental evidence.In: Sasaki S. Miyamoto T. Hopkin J.M. edsPrediction and Prevention of Childhood AllergyOsaka: Churchill‐Livingstone 1995:18197.
Carlotti D.N., 1992, Analysis of positive skin tests in 449 dogs with allergic dermatitis., Pratique Medicine Médicale et Chirougical de l’Animal de Compagnie, 27, 53
Koch H.J., 1994, 207 Intrtakutantests bei Hunden mit Verdacht auf atopische Dermatitis., Kleintierpraxis, 39, 25
Hammerling R., 1994, In‐vitro‐Vergleich des Allergengehalts von Hauttestlösungen verschiedener Anbieter im veterinärmedizinischen Bereich., Kleintierpraxis, 39, 687