Comparison of the Antigen Distribution of Two US Porcine Reproductive and Respiratory Syndrome Virus Isolates with that of the Lelystad Virus

Veterinary Pathology - Tập 33 Số 2 - Trang 159-170 - 1996
Patrick G. Halbur1, Prem S. Paul2, M. L. Frey3, John G. Landgraf3, K. A. Eernisse3, Xiang‐Jin Meng2, John J. Andrews1, Melissa A. Lum4, J. A. Rathje1
1Veterinary Diagnostic Laboratory
2Veterinary Medical Research Institute
3Iowa State University, Ames, IA; National Veterinary Services Laboratory, Ames, IA
4Solvay Animal Health, Mendota Heights, MN

Tóm tắt

One hundred 4-week-old cesarean-derived colostrum-deprived pigs were inoculated with one of two different US porcine reproductive and respiratory syndrome virus (PRRSV) isolates (VR2385, VR2431) or the European Lelystad virus to detect and compare the location and amount of virus antigen. Interstitial pneumonia, myocarditis, lymphadenopathy, and encephalitis were consistently seen in all three groups; however, disease and lesions were more severe in the VR2385 group. Immunohistochemical evaluation of formalin-fixed tissues revealed virus antigen in alveolar macrophages in lungs of 22/25, 14/25, 14/25, and 0/25 of the VR2385, VR2431, Lelystad, and control pigs, respectively. Follicular macrophages and dendritic cells in the lymph nodes of 14/25, 10/25, 10/25, and 0/25 pigs from the VR2385, VR2431, Lelystad, and control groups, respectively, stained positive for virus antigen. Similar cells in the tonsils from 25/25, 21/25, 23/25, and 0/25 pigs from the VR2385, VR2431, Lelystad, and control groups, respectively, stained positive for virus antigen. Other tissues and cells in which virus antigen was detected included macrophages and endothelial cells in the heart, macrophages, and interdigitating cells in the thymus, macrophages and dendritic cells in the spleen and Peyer's patches, and macrophages in hepatic sinusoids, renal medullary interstitium, and adrenal gland. PRRSV persisted in macrophages in the lung, tonsil, lymph node, and spleen for at least 28 days. Significantly more PRRSV antigen was detected in the lung ( P < 0.01), lymph nodes ( P ≤ 0.05), and tonsils ( P < 0.05) of the VR2385 pigs than was detected in the same tissues of the VR2431 and Lelystad pigs. The cell types in which PRRSV antigen was detected and the distribution of PRRSV antigen-positive cells within particular tissues and organs were generally similar for the different virus inoculation groups despite differences in virulence of the isolates.

Từ khóa


Tài liệu tham khảo

Abbas AK, 1994, Cellular and Molecular Immunology, 115

10.1177/104063879200400202

10.1177/104063879200400201

10.1136/vr.135.1.6

10.1136/vr.136.2.32

10.1136/vr.134.3.60

10.1177/104063879400600219

10.1177/030098589503200218

10.1136/vr.132.11.263

10.1177/030098589503200606

Hill H, 1990, Proc Mystery Swine Dis Comm Meet, 29

Huber SA, 1980, Am J Pathol, 98, 681

10.1007/BF01313785

10.1177/104063879400600302

Larochelle R, 1994, Can Vet J, 35, 513

Lydyard P, 1990, Immunology, 23

Magar R, 1993, Can J Vet Res, 57, 300

10.1099/0022-1317-75-7-1795

10.1007/BF01309962

Mengeling WL, 1994, Am J Vet Res, 55, 1391, 10.2460/ajvr.1994.55.10.1391

10.1006/viro.1993.1008

Molitor T, 1993, Leman Swine Conf, 49

Molitor T, 1992, Am Assoc Swine Pract Newsl, 4

10.1177/104063879400600402

10.1128/JCM.31.12.3184-3189.1993

10.1080/01652176.1991.9694298

Poison DD, 1994, Proc Congr Int Pig Vet Soc, 13, 436

Poison DD, 1992, Proc Congr Int Pig Vet Soc, 12, 132

10.1177/104063879400600102

10.1136/vr.134.16.422

10.1177/104063879200400203

10.1080/01652176.1991.9694296

Woodruff JF, 1980, Am J Pathol, 101, 425

10.1177/104063879200400205

10.1177/104063879400600326

10.1177/104063879300500404

Zhou Y, 1992, Am Assoc Swine Pract Newsl, 4

Thông tin
Thông tin xuất bản

Veterinary Pathology

Tập 33 Số 2

159-170

Thông tin tác giả