Comparison of monolithic silica and polymethacrylate capillary columns for LC

Journal of Separation Science - Tập 27 Số 10-11 - Trang 789-800 - 2004
Dana Moravcová1, Pavel Jandera, Jiřı́ Urban1, Josef Planeta2
1Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Nám. Čs. Legií 565, 532 10 Pardubice, Czech Republic; Phone: 420 46 6037023; Fax: 420 46 6037068
2Institute of Analytical Chemistry, Czech Academy of Sciences, Veveří 97, 611 42 Brno, Czech Republic

Tóm tắt

AbstractOrganic polymer monolithic capillary columns were prepared in fused‐silica capillaries by radical co‐polymerization of ethylene dimethacrylate and butyl methacrylate monomers with azobisisobutyronitrile as initiator of the polymerization reaction in the presence of various amounts of porogenic solvent mixtures and different concentration ratios of monomers and 1‐propanol, 1,4‐butanediol, and water. The chromatographic properties of the organic polymer monolithic columns were compared with those of commercial silica‐based particulate and monolithic capillary and analytical HPLC columns. The tests included the determination of H‐u curves, column permeabilities, pore distribution by inversed‐SEC measurements, methylene and polar selectivities, and polar interactions with naphthalenesulphonic acid test samples. Organic polymer monolithic capillary columns show similar retention behaviour to chemically bonded alkyl silica columns for compounds with different polarities characterized by interaction indices, Ix, but have lower methylene selectivities and do not show polar interactions with sulphonic acids. The commercial capillary and analytical silica gel‐based monolithic columns showed similar selectivities and provided symmetrical peaks, indicating no significant surface heterogeneities. To allow accurate characterization of the properties of capillary monolithic columns, the experimental data should be corrected for extra‐column contributions. With 0.3 mm ID capillary columns, corrections for extra‐column volume contributions are sufficient, but to obtain true information on the efficiency of 0.1 mm ID capillary columns, the experimental bandwidths should be corrected for extra‐column contributions to peak broadening.

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Journal of Separation Science

Tập 27 Số 10-11

789-800

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