Comparison of Neutralizing and Hemagglutination-Inhibiting Antibody Responses to Influenza A Virus Vaccination of Human Immunodeficiency Virus-Infected Individuals

American Society for Microbiology - Tập 5 Số 1 - Trang 114-117 - 1998
C. A. Benne1,2, Frank P. Kroon3, Martin C. Harmsen4, Luís Tavares4, C. A. Kraaijeveld4, J.C. de Jong5
1Eijkman-Winkler Laboratory of Medical Microbiology, University Hospital Utrecht,1; Regional Public Health Laboratory Groningen, Groningen,2The Netherlands
2Regional Public Health Laboratory Groningen, Groningen,2The Netherlands
3Department of Infectious Diseases, University Hospital Leiden, Leiden,3
4Eijkman-Winkler Laboratory of Medical Microbiology, University Hospital Utrecht,1
5Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, Bilthoven,4 and

Tóm tắt

ABSTRACT

A neutralization enzyme immunoassay (N-EIA) was used to determine the neutralizing serum antibody titers to influenza A/Taiwan/1/86 (H1N1) and Beijing/353/89 (H3N2) viruses after vaccination of 51 human immunodeficiency virus (HIV) type 1-infected individuals and 10 healthy noninfected controls against influenza virus infection. Overall, the N-EIA titers correlated well with the hemagglutination-inhibition (HAI) titers that were observed in the same samples in a previous study (F. P. Kroon, J. T. van Dissel, J. C. de Jong, and R. van Furth, AIDS 8:469–476,1994). The N-EIA appeared to be more sensitive than the HAI test. Significantly more fourfold or higher rises in N-EIA titer and higher mean N-EIA titers occurred in HIV-infected individuals with ≥200 CD4 + cells per μl than in those with <200 CD4 + cells per μl.

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