Comparison of Fatty Acid Analysis with Serotype and Pulsed‐Field Gel Electrophoresis for Typing Salmonella Isolated from Retail Foods and Human

Journal of Food Safety - Tập 34 Số 4 - Trang 388-395 - 2014
Pengfei Zhang1,2, Xiaonan Xing3, Xin Wang3, Baowei Yang3, Chengyu Fu2, Liu De-hao2, Chunfeng Guo3
1Northwestern Polytechnical University, Xi’an, Shaanxi, China
2Shaanxi Entry-Exit Inspection and Quarantine Bureau, Xi'an, Shaanxi, China
3College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China

Tóm tắt

AbstractWhole‐cell fatty acid analysis by pyrolysis‐gas chromatography/mass spectrometry was compared with pulsed‐field gel electrophoresis (PFGE) and serotyping to determine the best suited method for identification of Salmonella strains. Forty‐two isolates were analyzed and 10 fatty acids were identified in all of the tested strains. The main fatty acid component was n‐hexadecanoic acid (C16:0), followed by cis‐9‐hexadecenoic acid (C16:1ω‐7c), cis‐10‐heptadecenoic acid (C17:1ω‐7c), tetradecanoic acid (C14:0), cis‐10‐nonadecenoic acid (C19:1ω‐9c), trans‐2‐dodecenoic acid (C12:1ω‐10t), cis‐vaccenic acid (C18:1ω‐8c), dodecanoic acid (C12:0), octadecanoic acid (C18:0) and 2‐tridecanoic acid (E)‐(C13:0). Whole‐cell fatty acid analysis, PFGE and serotyping yielded 38, 35 and 29 types, respectively. Simpson's diversity indices of the three typing methods were 0.994, 0.985 and 0.943, respectively. PFGE grouped all the strains into 27 clusters based on 80% similarity. Fatty acid analysis was grouped into four clusters based on r = 0.8. Our findings indicate that whole‐cell fatty acid analysis was found to help with rapid identification of Salmonella strains. Whole‐cell fatty acid analysis was not only as portable as PFGE and serotyping but also more rapid and cost‐effective.Practical ApplicationsFoodborne diseases caused by Salmonella enterica represent an important public health problem worldwide. Therefore, tracing sporadic cases and outbreaks of Salmonella infection is important for epidemiological purposes. In this context, fatty acid analysis is a rapid and cost‐effective method for identification and classification of Salmonella strains, and it can help expedite the process of foodborne outbreak investigation.

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Tài liệu tham khảo

10.1016/S0167-7012(97)00038-9

Chen J., 2010, Analysis of the fatty acid components of Brucella strains in Guangdong province, Chin. J. Zoonoses, 26, 131

10.1128/AEM.56.6.1717-1724.1990

10.1016/S0168-1605(02)00323-9

Guo A.L., 2007, Studies on the whole cell of Salmonella by pyrolysis‐gas chromatography‐mass spectrometry, Chin. J. Anal. Chem., 35, 700

10.1128/JCM.26.11.2465-2466.1988

Li J., 2004, Analysis of genomic DNA and cellular fatty acid composition of Nocardia farcinica, China J. Lepr. Skin Dis., 20, 418

10.1016/j.jaap.2008.02.002

10.1016/j.mimet.2007.01.013

Qin Q., 2008, GC‐MS analysis of fatty acid from whole cell of Shigella, Chin. J. Health Lab. Technol., 18, 426

10.1128/JCM.00420-06

10.1111/j.1574-695X.2007.00305.x

10.1016/j.mimet.2003.07.005

Wu M., 2006, The analysis of fatty acid component of Yersinia pestis strains from Yunnan, Chin. J. Control Endem. Dis., 21, 71

Yan L., 1997, Gas chromatographic whole‐cell fatty acid analysis and its application for the identification of Ps. aeruginosa, Chin. J. Nosocomiol., 7, 7

10.1016/j.ijfoodmicro.2010.04.015

Yang C., 2012, Cellular fatty acids as chemical markers for differentiation of Acinetobacter baumannii and Acinetobacter calcoaceticus, Biomed. Environ. Sci., 25, 711

Zhao H., 2008, Analysis of fatty acid from Listeria monocytogenes by gas chromatography‐ mass spectrometry, Chem. Bioengin., 25, 68

10.1128/JCM.00645-10

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Thông tin xuất bản

Journal of Food Safety

Tập 34 Số 4

388-395

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