Sophie Paris1, Catherine Fitting2, Jean‐Paul Latgé1, Dale E. Herman3, M.T. Guinnepain4, Brinda G. David2
1Unité de Mycologie, et
2Unité immunoallergie, Institut Pasteur, et
3Hôpital Lariboisière, et
4Unité allergologie, Institut Pasteur, Paris, France
Tóm tắt
<i>Alternaria allergens</i>, separated by SDS polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane, were identified using sera from <i>Alternaria</i>-allergic patients. 30 <i>Alternaria</i> components of spore and mycelium, ranging in molecular weight from more than 150 to 12 kD, bound IgE antibodies. 15 were detected by more than 25% of the sera and only 4 (85, 56, 42, 31 kD) by more than 50% of the sera. Among these four major allergens, the 56-kD component was present mostly in the spore extract, the 85- and 42-kD fractions in the mycelium extract and the 31-kD in both extracts. The 31-kD component showed the highest IgE binding and the highest frequency of binding (95% with mycelium extract and 79% with spore extract). It was composed of two subunits.
