So sánh và định lượng HPLC của hồ sơ chống oxy hóa từ chiết xuất củ gừng, lá gừng và hoa gừng

Saira Tanweer1, Tariq Mehmood1, Saadia Zainab2, Zulfiqar Ahmad3, Aamir Shehzad1
1National institute of food Science and technology, University of Agriculture Faisalabad, Faisalabad, Pakistan
2Department of Food Engineering, University of Agriculture Faisalabad, Faisalabad, Pakistan
3Department of Food Science and Technology, The Islamia University of Bahawalpur, Bahawalpur, Pakistan

Tóm tắt

Tóm tắt Đặt vấn đề Trong thời đại hiện nay, sự chú ý của các chuyên gia dinh dưỡng đã chuyển hướng sang các thực thể sinh học có lợi có trong các nguồn tự nhiên do khả năng tăng cường sức khỏe chống lại các rối loạn liên quan đến lối sống. Phương pháp Trong bối cảnh này, các bộ phận khác nhau của cây gừng, tức là củ, lá và hoa của giống Suravi (ID số 008) đã được sử dụng để chuẩn bị các chiết xuất gừng với 50% methanol, 50% ethanol và nước thông qua máy lắc quay trong 45 phút. Sau đó, các phân tích phytochemical khác nhau và các phân tích in vitro đã được thực hiện để xác định khả năng chống oxy hóa của các chiết xuất này. Cuối cùng, các chiết xuất được chọn lọc tốt nhất từ mỗi bộ phận đã được định lượng thông qua HPLC. Kết quả Kết quả điều tra hiện tại cho thấy chiết xuất ethanol có lượng phytoceutics tối đa so với methanol và nước. Tổng hàm lượng polyphenol (TPC), flavonoid, flavonol, thử nghiệm DPPH, hoạt động chống oxy hóa, thử nghiệm FRAP, thử nghiệm ABTS và khả năng chelat kim loại cao nhất được quan sát trong lá gừng với các giá trị lần lượt là 780.56 ± 32.78 GAE/100 g, 253.56 ± 10.65 mg/100 g, 49.54 ± 1.74 mg/100 g, 75.54 ± 3.17%, 77.88 ± 3.27%, 105.72 ± 4.44 μmole TE/g, 118.43 ± 4.97 μmole TE/g và 35.16 ± 1.48%. Theo sau đó là hoa gừng và củ gừng. Hoạt động chống oxy hóa thấp nhất được ước tính ở củ gừng. Dựa trên hồ sơ phytochemical và các phân tích in vitro, các chiết xuất ethanol từ hoa gừng, lá gừng và củ gừng đã được chọn để định lượng thông qua HPLC. Kết luận Những phát hiện cho thấy rằng hàm lượng 6-gingerol cao nhất có trong lá gừng (4.9 mg/g), tiếp theo là hoa gừng (2.87 mg/g) và củ gừng (1.03 mg/g).

Từ khóa


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