Comparative study on general properties of alginate lyases from some marine gastropod mollusks
Tóm tắt
Alginate lyase (EC 4.2.2.3) is an enzyme that splits glycosyl linkages of alginate chain via β-elimination, producing unsaturated oligoalginates. This enzyme is widely distributed in herbivorous marine mollusks, brown algae, and marine and soil bacteria. In the present study, we determined the general properties and partial amino acid sequences of alginate lyases from three Archeogastropoda, i.e., Haliotis discus hannai, H. iris, and Omphalius rusticus, and one Mesogastropoda, i.e., Littorina brevicula, in order to enrich the information about functional and structural diversity in gastropod alginate lyases. The alginate lyases were extracted from hepatopancreas of these animals and purified by ammonium sulfate fractionation followed by conventional column chromatography. Single alginate lyases with molecular masses of approximately 28, 34, and 34 kDa were isolated from H. discus, H. iris, and O. rusticus, respectively. While three alginate lyases with molecular masses of 35, 32, and 28 kDa were isolated from L. brevicula. These enzymes were identified as poly(M) lyase (EC 4.2.2.3) since they preferably degraded poly(M)-rich substrate. Western blot analysis using an antiserum raised against H. discus enzyme suggested that H. iris, and O. rusticus enzymes shared similar primary/higher-order structure with H. discus enzyme, but the L. brevicula enzymes did not. H. discus, H. iris, and O. rusticus enzymes were classified to polysaccharide-lyase family-14 by the analysis of partial amino acid sequences, while the L. brevicula enzymes were not.
Tài liệu tham khảo
Haug A, Larsen B, Smidsrød O (1967) Studies on the sequence of uronic acid residues in alginic acid. Acta Chem Scand 21:691–704
Gacesa P (1988) Alginates. Carbohydr Polym 8:161–182
Gacesa P (1992) Enzymatic degradation of alginates. Int J Biochem 24:545–552
Wong TY, Preston LA, Schiller NL (2000) Alginate lyase: review of major sources and enzyme characteristics, structure-function analysis, biological roles, and applications. Annu Rev Microbiol 54:289–340
Draget KI, Smidsrod O, Skjak-Braek G (2002) Alginates from algae. In: Beats SD, Vandamme EJ, Steinbuchel A (eds) Biopolymer, vol 6, Polysaccharides II. Wiley-VCH, Germany, pp 215–244
Tomoda Y, Umemura K, Adachi T (1994) Promotion of barley root elongation under hypoxic conditions by alginate lyase lysate. Biosci Biotechnol Biochem 58:202–203
Sutherland IW (1995) Polysaccharide lyases. FEMS Microbiol Rev 16:323–347
Xu X, Iwamoto Y, Kitamura Y, Oda T, Muramatsu T (2003) Root growth-promoting activity of unsaturated oligomeric uronates from alginate on carrot and rice plants. Biosci Biotechnol Biochem 67:2022–2025
Akiyama H, Endo T, Nakakita R, Murata K, Yonemoto Y, Okayama K (1992) Effect of depolymerized alginates on the growth of bifidobacteria. Biosci Biotechnol Biochem 56:355–356
Natsume M, Kamo Y, Hirayama M, Adachi T (1994) Isolation and characterization of alginate-derived oligosaccharides with root growth-promoting activities. Carbohydr Res 258:187–197
Iwamoto Y, Xu X, Tamura T, Oda T, Muramatsu T (2003) Enzymatically depolymerized alginate oligomers that cause cytotoxic cytokine production in human mononuclear cells. Biosci Biotechnol Biochem 67:258–263
Yoshida T, Hirano A, Wada H, Takahashi K, Hattori M (2004) Alginic acid oligosaccharide suppresses Th2 development and IgE production by inducing IL-12 production. Int Arch Allergy Immunol 133:239–247
Tsuchida T, Chaki T, Ogawa H (2001) Effect of sodium alginate oligosaccharide on human blood pressure. Med Cons New-Remed 38:555–560 (in Japanese)
Chaki T, Nishimoto S, Hiura N, Satou R, Tou Y, Kakinuma S (2002) Effect of a powdered drink containing sodium alginate oligosaccharide on blood pressure in mild hypertensive and high normal blood pressure subjects. J Nutr Food 5:41–54 (in Japanese)
Nakada HI, Sweeny PC (1967) Alginic acid degradation by eliminases from abalone hepatopancreas. J Biol Chem 242:845–851
Nishizawa K, Fujibayashi S, Kashiwabara Y (1968) Alginate lyases in the hepatopancreas of a marine mollusc, Dolabella auricula Solander. J Biochem (Tokyo) 64:25–37
Elyakova LA, Favarov VV (1974) Isolation and certain properties of alginate lyase VI from the mollusk Littorina sp. Biochim Biophys Acta 358:341–354
Muramatsu T, Hirose S, Katayose M (1977) Isolation and properties of alginate lyase from the mid-gut gland of wreath shell Turbo cornutus. Agric Biol Chem 41:1939–1946
Boyen C, Kloareg B, Polne-Fuller M, Gibor A (1990) Preparation of alginate lyases from marine molluscs for protoplast isolation in brown algae. Phycologia 29:173–181
Heyraud A, Colin-Morel P, Girond S, Richard C, Kloareg B (1996) HPLC analysis of saturated or unsaturated oligoguluronates and oligomannuronates. Application to the determination of the action pattern of Haliotis tuberculata alginate lyase. Carbohydr Res 291:115–126
Muramatsu T, Komori K, Sakurai N, Yamada K, Awasaki Y, Fukuda K, Oda T (1996) Primary structure of mannuronate lyases SP1 and SP2 from Turbo cornutus and involvement of the hydrophobic C-terminal residues in the protein stability. J Protein Chem 15:709–719
Shimizu E, Ojima T, Nishita K (2003) cDNA cloning of an alginate lyase from abalone, Haliotis discus hannai. Carbohydr Res 338:2841–2852
Suzuki H, Suzuki K, Inoue A, Ojima T (2006) A novel oligoalginate lyase from abalone, Haliotis discus hannai, that releases disaccharide from alginate polymer in an exolytic manner. Carbohydr Res 341:1809–1819
Madgwick J, Haug A, Larsen B (1973) Alginate lyase in the brown alga Laminaria digitata (Huds.). Lamour Acta Chem Scand 27:711–712
Watanabe T, Nishizawa K (1982) Enzymatic studies on alginate lyase from Undaria pinnatifida in relation to texture-softening prevention by ash-treatment (Haiboshi). Bull Jpn Soc Sci Fish 48:243–249
Murata K, Inose T, Hisano T, Abe S, Yonemoto Y, Yamashita T, Takagi M, Sakaguchi K, Kumura A, Imanaka T (1993) Bacterial alginate lyase: enzymology, genetics and application. J Ferment Bioeng 76:427–437
Sawabe T, Ohtsuka M, Ezura Y (1997) Novel alginate lyases from marine bacterium Alteromonas sp. strain H-4. Carbohydr Res 28:69–76
Sugimura II, Sawabe T, Ezura Y (2000) Cloning and sequence analysis of Vibrio halioticoli genes encoding three types of polyguluronate lyase. Mar Biotechnol 2:65–73
Iwamoto Y, Araki R, Iriyama K, Oda T, Fukuda H, Hayashida S, Muramatsu T (2001) Purification and characterization of bifunctional alginate lyase from Alteromonas sp. strain no. 272 and its action on saturated oligomeric substrates. Biosci Biotechnol Biochem 65:133–142
Zhang Z, Yu G, Guan H, Zhao X, Du Y, Jiang X (2004) Preparation and structural elucidation of alginate oligosaccharides degraded by alginate lyase from Vibrio sp. 510. Carbhydr Res 339:1475–1481
Suda K, Tanji Y, Hori K, Unno H (1996) Evidence for a novel Chlorella virus-encoded alginate lyase. FEMS Microbiol Lett 180:45–53
Seiderer LJ, Newell RC, Cook PA (1982) Quantitative significance of style enzymes from two marine mussels Choromytilus meridionalis and Perna perna in relation to diet. Mar Biol Lett 3:257–272
Chaki T, Kakimi H, Shibata A, Baba T (2006) Detection of alginate oligosaccharides from mollusks. Biosci Biotechnol Biochem 70:2793–2796
Gasesa P, Wusteman FS (1990) Plate assay for simultaneous detection of alginate lyases and determination of substrate specificity. Appl Environ Microbiol 56:2265–2267
Porzio MA, Pearson AM (1977) Improved resolution of myofibrillar proteins with sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Biochem Biophys Acta 490:27–34
Towbin H, Staehelim T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
Gornall AG, Bardawill CJ, David MM (1949) Determination of serum proteins by means of the biuret reaction. J Biol Chem 177:751–766
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275