Comparative analysis of gene expression under cold acclimation, deacclimation and reacclimation in Arabidopsis

Physiologia Plantarum - Tập 152 Số 2 - Trang 256-274 - 2014
Youn‐Jung Byun1, Mwl Koo1, Hye‐Joon Joo1, Young‐Mie Ha‐Lee2, Dong‐Hee Lee1
1Graduate Department of Life and Pharmaceutical Science, Ewha Womans University, Seoul, 120-750, South Korea.
2Institute for Scientifically Able Youth Gachon University Seongnam 461‐701 South Korea

Tóm tắt

Cold acclimated plants show an elevated tolerance against subsequent cold stress. Such adaptation requires alterations in gene expression as well as physiological changes. We were interested in gene expression changes at the transcriptional level during adaptation processes. The patterns of transcriptional changes associated with cold acclimation, deacclimation and reacclimation in Arabidopsis leaves were characterized using the Coldstresschip. Gene expression profiles were further analyzed by ‘coexpressed gene sets’ using gene set enrichment analysis (GSEA). Genes involved in signal transduction through calcium, and cascades of kinases and transcription factor genes, were distinctively induced in the early response of cold acclimation. On the other hand, genes involved in antioxidation, cell wall biogenesis and sterol synthesis were upregulated in the late response of cold acclimation. After the removal of cold, the expression patterns of most genes rapidly returned to the original states. However, photosynthetic light‐harvesting complex genes and lipid metabolism‐related genes stayed upregulated in cold deacclimated plants compared to non‐treated plants. It is also notable that many well‐known cold‐inducible genes are slightly induced in reacclimation and their expression remains at relatively low levels in cold reacclimation compared to the expression during the first cold acclimation. The results in this study show the dynamic nature of gene expression occurring during cold acclimation, deacclimation and reacclimation. Our results suggest that there is a memory of cold stress and that the ‘memory of cold stress’ is possibly due to elevated photosynthetic efficiency, modified lipid metabolism, increased calcium signaling, pre‐existing defense protein made during first cold acclimation and/or modified signal transduction from pre‐existing defense protein.

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Tài liệu tham khảo

10.1016/S0092-8674(00)81405-1

10.1111/j.2517-6161.1995.tb02031.x

10.1016/S1369-5266(98)80115-5

10.1073/pnas.0406389101

10.1007/s00425-009-0903-9

10.1093/bioinformatics/btp502

10.1126/science.278.5338.680

10.1104/pp.110.154815

10.1038/4434

10.1111/j.1399-3054.2006.00627.x

10.1007/BF03031132

10.1105/tpc.003483

10.1016/j.pbi.2008.09.007

10.1073/pnas.98.1.373

10.1046/j.1365-313X.2003.01872.x

10.1093/pcp/pci136

10.1371/journal.pgen.0010026

10.1104/pp.109.2.697

10.1007/s12374-012-0382-6

10.1101/gr.184401

10.1016/j.plantsci.2006.02.013

10.1126/science.1061603

10.1007/s10535-009-0088-x

10.1109/5.58325

10.1006/eesa.1998.1668

10.1104/pp.103.020511

10.1101/gr.1910904

10.1105/tpc.105.035568

10.1111/j.1749-6632.1998.tb08993.x

10.1002/dvg.1020070402

10.1073/pnas.0913759107

10.1038/nature05022

10.1007/BF00396344

10.1007/s10142-005-0014-z

10.1146/annurev.arplant.54.072402.115741

10.1016/S0006-2952(03)00504-5

10.1093/aob/mcf090

10.1034/j.1399-3054.2002.1150209.x

10.1104/pp.103.022939

10.2174/138920211794520178

10.1186/gb-2013-14-6-r59

10.1104/pp.103.024018

10.1007/0-387-29362-0_23

10.1046/j.1365-313X.1997.00583.x

10.1104/pp.119.4.1387

10.1073/pnas.0506580102

10.1093/treephys/26.3.325

10.1016/j.lungcan.2011.05.028

Team RDC(2012) R: A language and environment for statistical computing. R Foundation for Statistical Computing Vienna Austria. Available athttp://www.R‐project.org/(accessed 19 November 2013)

10.1111/j.1365-313X.2004.02016.x

10.1104/pp.118.1.1

10.1016/j.plantsci.2007.09.002

10.1016/0958-1669(92)90147-B

10.1016/j.plaphy.2008.06.006

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Physiologia Plantarum

Tập 152 Số 2

256-274

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