Comparative Transcriptome and Secretome Analysis of Wood Decay Fungi Postia placenta and Phanerochaete chrysosporium

Applied and Environmental Microbiology - Tập 76 Số 11 - Trang 3599-3610 - 2010
Amber Vanden Wymelenberg1, Jill Gaskell2, Michael D. Mozuch2, Grzegorz Sabat3, John Ralph4, Oleksandr Skyba5, Shawn D. Mansfield5, Robert A. Blanchette6, Diego Martínez7, Igor V. Grigoriev8, Philip J. Kersten2, Dan Cullen2
1Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706,
2USDA, Forest Service, Forest Products Laboratory, Madison, Wisconsin, 53726
3Genetics and Biotechnology Center, University of Wisconsin, Madison, Wisconsin 53706
4Department of Biochemistry and Department of Energy, Great Lakes Bioenergy Research Center, University of Wisconsin, Madison, Wisconsin 53726
5Department of Wood Science, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
6Department of Plant Pathology, University of Minnesota, St. Paul, Minnesota, 55108
7Department of Biology, University of New Mexico, Albuquerque, New Mexico, 87131
8Department of Energy Joint Genome Institute, Walnut Creek, California 94598

Tóm tắt

ABSTRACT Cellulose degradation by brown rot fungi, such as Postia placenta , is poorly understood relative to the phylogenetically related white rot basidiomycete, Phanerochaete chrysosporium . To elucidate the number, structure, and regulation of genes involved in lignocellulosic cell wall attack, secretome and transcriptome analyses were performed on both wood decay fungi cultured for 5 days in media containing ball-milled aspen or glucose as the sole carbon source. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), a total of 67 and 79 proteins were identified in the extracellular fluids of P. placenta and P. chrysosporium cultures, respectively. Viewed together with transcript profiles, P. chrysosporium employs an array of extracellular glycosyl hydrolases to simultaneously attack cellulose and hemicelluloses. In contrast, under these same conditions, P. placenta secretes an array of hemicellulases but few potential cellulases. The two species display distinct expression patterns for oxidoreductase-encoding genes. In P. placenta , these patterns are consistent with an extracellular Fenton system and include the upregulation of genes involved in iron acquisition, in the synthesis of low-molecular-weight quinones, and possibly in redox cycling reactions.

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Applied and Environmental Microbiology

Tập 76 Số 11

3599-3610

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